【摘要】 目的:建立HPLC法同时测定中亚鸢尾中3种具有抗炎活性的黄酮类成分4’-羟基-5,3’-二甲氧基-6,7-亚甲二氧基异黄酮-4’-O-β-D-吡喃葡萄糖苷、尼鸢尾黄素-4’-O-β-D-吡喃葡萄糖苷、4’-羟基-5,3’-二甲氧基-6,7-亚甲二氧基异黄酮含量。方法:色谱柱为HC-C₁₈柱（250 mm×4.6 mm,5μm）,流动相为乙腈-0.3%磷酸溶液,流速:1.0 ml·min^-1,检测波长:265 nm,柱温:25℃,进样量:5μl。结果:4’-羟基-5,3’-二甲氧基-6,7-亚甲二氧基异黄酮-4’-O-β-D-吡喃葡萄糖苷、尼鸢尾黄素-4’-O-β-D-吡喃葡萄糖苷、4’-羟基-5,3’-二甲氧基-6,7-亚甲二氧基异黄酮质量浓度分别在5.25～84.00μg·ml^-1（r=0.999 5）、3.25～52.00μg·ml^-1（r=0.999 6）和3.25～52.00μg·ml^-1（r=0.999 8）的范围内与峰面积呈良好的线性关系;平均加样回收率分别为98.07%（RSD=1.58%）、98.65%（RSD=2.17%）和97.21%（RSD=3.46%）（n=9）。结论:该方法准确度好,精密度高,可用于中亚鸢尾中3种黄酮类成分的同时测定。更多还原

【Abstract】 Objective: To establish an HPLC method for the simultaneous determination of three flavonoids with anti-inflammatory activity in Iris bloudowii. Methods: The method was performed on an HC-C₁₈ column （250 mm×4.6 mm,5 μm） with the mobile phase consisting of acetonitrile-0.3% phosphoric acid solution with gradient elution at the flow rate of 1.0 ml·min^-1,the detection wavelength was 265 nm,the column temperature was 25℃ and the sample size was 5 μl. Results: The linear range of nigricanin-4’-O-β-D-glucoside,nigricin-4’-O-β-D-glucoside and nigricanin was 5.25-84.00 μg · ml^-1 （r = 0.999 5）,3.25-52.00 μg·ml^-1 （r = 0.999 6） and 3.25-52.00 μg·ml^-1 （r = 0.999 8）,respectively. The average recovery was 98.07% （RSD = 1.58%）,98.65% （RSD = 2.17%） and97.21% （RSD = 3.46%） （n = 9）,respectively. Conclusion: The method is accurate with high precision,which can be applied to the simultaneous determination of three flavonoids in Iris bloudowii.更多还原