【摘要】 目的:探讨PI3K/Akt信号通路相关蛋白PI3K、Akt、PTEN在胆管癌(cholangiocarcinoma,CCA)中的表达及其临床意义。方法:收集2010年1月-2017年1月本院47例CCA患者的癌组织及对应癌旁组织标本,另选择同期14例正常胆管组织标本。比较CCA、癌旁组织及正常胆管组织中PI3K、Akt与PTEN蛋白的阳性表达率,并分析PI3K、Akt与PTEN蛋白在CCA组织中阳性表达的相关因素及相关性。结果:CCA组织中PI3K与Akt的阳性表达率均高于癌旁组织和正常胆管组织(P<0.05);CCA组织中PTEN阳性表达率均低于癌旁组织和正常胆管组织(P<0.05);CCA组织、癌旁组织及正常胆管组织的PI3K、Akt及PTEN阳性表达率比较,差异均有统计学意义(P<0.05)。有无神经侵犯、浸润深度、淋巴结转移情况及TNM分期是PI3K、Akt及PTEN蛋白在CCA组织中阳性表达的相关因素(P<0.05)。PI3K、Akt与PTEN在CCA组织中的表达呈负相关(r=-0.635、-0.506,P<0.001),而PI3K与Akt在CCA组织中的表达呈正相关(r=0.332,P=0.023)。结论:PI3K/Akt信号传导通路在CCA组织中过度表达,可能参与了CCA的发生与发展,可作为生物学行为的评估指标,PI3K/Akt信号通路可望成为CCA临床干预的分子靶点。更多还原

【Abstract】 Objective:To investigate the expression and clinical significance of protein PI3K,Akt and PTEN which correlated with PI3K/Akt signaling pathway in cholangiocarcinoma (CCA).Method:Cancer tissues and corresponding paracancer tissue specimens of 47 CCA patients were collected from our hospital from January 2010 to January 2017.Another 14 normal bile duct specimens were selected.The positive expression rates of PI3K,Akt and PTEN proteins in CCA,paracancer and normal bile duct tissues were compared,and the relevant factors and correlations of PI3K,Akt and PTEN proteins expressions in CCA tissues were analyzed.Result:The positive expression rates of PI3K and Akt in CCA tissues were higher than those in paracancer and normal bile duct tissues (P<0.05).The positive expression rate of PTEN in CCA tissues was lower than that in paracancer and normal bile duct tissues (P<0.05).There were significant differences in the positive expression rates of PI3K,Akt and PTEN in CCA,paracancer and normal bile duct tissues (P<0.05).The presence of nerve invasion,depth of infiltration,lymph node metastasis and TNM stage were the relevant factors for the positive expression of PI3K,Akt and PTEN in CCA tissues (P<0.05).PI3K and Akt were negatively correlated with the expression of PTEN in CCA tissues (r=-0.635,-0.506,P<0.001),while PI3K was positively correlated with the expression of Akt in CCA tissues (r=0.332,P=0.023).Conclusion:The PI3K/Akt signaling pathway is overexpressed in CCA tissues,which may be involved in the occurrence and development of CCA,and can be used as an evaluation indicator of biological behavior,PI3K/Akt signaling pathway is expected to become a molecular target for clinical intervention of CCA.更多还原