【摘要】 目的探讨热疗联合顺铂对喉癌Hep-2细胞的影响,了解热疗与顺铂的治疗方法是否有交互作用。方法将传代的Hep-2细胞分为对照组、热疗组、顺铂组及热疗联合顺铂组。对照组置于37℃培养箱,热疗组置于43℃培养箱,顺铂组置于37℃培养箱并加入顺铂,热疗联合顺铂组置于43℃培养箱并加入顺铂。采用MTT法、Wound-healing划痕实验和Transwell侵袭实验检测热疗联合顺铂对Hep-2细胞的增殖、迁移和侵袭力影响。结果 4组细胞在波长490 nm处光密度(OD)值分别为(1.24±0.13)、(0.99±0.11)、(0.75±0.07)及(0.31±0.07),析因分析显示,不同温度热疗在波长490 nm处的OD值有差异(P <0.05),不同浓度顺铂在波长490 nm处的OD值有差异(P <0.05),热疗与顺铂对在波长490 nm处的OD值存在交互作用(P <0.05)。4组细胞未愈合区百分比分别为(31.83±1.62)%、(40.47±1.52)%、(53.13±2.61)%及(79.83±0.95)%,析因分析显示,不同温度热疗对未愈合区百分比有差异(P <0.05),不同浓度顺铂未愈合区百分比有差异(P <0.05),热疗与顺铂对未愈合区百分比的影响存在交互作用(P <0.05)。4组细胞穿过Transwell小室膜的细胞数目分别为(27.8±1.64)、(21.8±1.48)、(11.2±1.48)及(1.8±0.84)个,析因分析显示,不同温度热疗的穿膜细胞数有差异(P <0.05),不同浓度顺铂的穿膜细胞数有差异(P <0.05),热疗与顺铂对穿膜细胞数的影响存在交互作用(P <0.05)。结论热疗能够增强喉癌Hep-2细胞对顺铂的敏感性,热疗与顺铂联合有协同抗肿瘤作用。更多还原

【Abstract】 Objective To investigate the effect of proliferation and invasion on hyperthermia combinated with cisplatin in human laryngeal carcinoma Hep-2 cells and whether the two treatments have an interaction.Methods The Hep-2 cells were randomly divided into 4 groups:control group (37℃),hyperthermia group (43℃),cisplatin group and hyperthermia combined with cisplatin group.The effects of hyperthermia combined with cisplatin on proliferation,migration and invasion of Hep-2 cells were determined by MTT assay,Wound-healing assay and transwell invasion assay.Results Four groups of cells were administrated with MTT.The OD value at 490 nm were (1.24±0.13),(0.99±0.11),(0.75±0.07) and (0.31±0.07) respectively.Factorial analysis showed that OD values at different temperatures were statistically significant (P < 0.05).OD values at different concentrations of cisplatin were statistically significant (P < 0.05).There was an interaction between hyperthermia and cisplatin on OD value at 490 nm(P < 0.05).The percentage of ’unhealed area’ in the four groups were (31.83±1.62) %,(40.47±1.52) %,(53.13±2.61) % and (79.83±0.95) % respectively.Factorial analysis showed that the difference of the influence of different temperature of hyperthermia on the percentage of ’unhealed area’ was statistically significant (P < 0.05).The influence of different concentrations of cisplatin on the percentage of ’unhealed area’ was statistically significant (P < 0.05).There was an interaction between the influence of hyperthermia and cisplatin on the percentage of ’unhealed area’ (P < 0.05).The number of invasion cells in the four groups were (27.80±1.64),(21.80±1.48),(11.20±1.48) and (1.80±0.84) respectively.Factorial analysis showed that the influence of different temperature of hyperthermia on the number of invasion cells was statistically significant (P < 0.05).The influence of different concentrations of cisplatin on the number of invasion cells was statistically significant (P < 0.05).There was an interaction between the effects of hyperthermia and cisplatin on the number of invasion cells (P < 0.05).Conclusion Hyperthermia can enhance the sensitivity of Hep-2 cells to cisplatin.Hyperthermia combined with cisplatin has synergistic anti-tumor effects.更多还原