【摘要】 以一年生实生山桃苗为试材,通过正常培养、干旱胁迫以及干旱胁迫下外施脱落酸(ABA)的山桃叶片进行高通量转录组测序分析,探究山桃响应干旱胁迫的分子机制,筛选响应ABA处理的功能基因。结果表明:试验共构建9个文库进行转录组测序。转录组测序共获得67.14 Gb Clean Data,各样品Clean Data均达到5.81 Gb,Q30碱基百分比在91.71%及以上。与对照相比,干旱处理组差异表达基因显著上调596个,下调1 025个。与干旱处理组相比,干旱+ABA处理组差异表达基因显著上调394个,下调117个。GO富集分析表明:对照组、干旱处理组和干旱+ABA处理组在生物学途径中富集最多的类别都是代谢过程;在细胞组件和分子功能中, 3个不同处理组富集最多的基因类别均是细胞组分和催化活性相关的基因。KEGG富集分析表明:与对照相比,干旱处理组的样本有260个差异表达基因参与72条代谢途径;与干旱处理组相比,干旱+ABA处理组的样本有66个差异表达基因参与了38条代谢途径。经KEGG显著性富集分析,筛选到淀粉和蔗糖代谢、植物激素信号转导等相关基因。采用实时荧光定量PCR对筛选到的基因进行了表达检测,其趋势与转录组分析结果基本一致。更多还原

【Abstract】 IlluminaHiSeqTM 4000, a high-through transcriptome sequencing technology, was applied to obtain the transcriptome differential expression data of David peach(Amygdalus davidiana) leaves under normal culture, drought stress and exposed to abscisic acid(ABA) under drought stress, to understand the inner molecular mechanisms of David peach and the functional genes related to ABA in response to drought stress. The results showed that the transcriptional sequence of 9 samples was completed. Transcriptome sequencing was used to obtain 67.14 GB Clean Data, and the Clean Data of all samples reached 5.81 GB, and the percentage of Q30 bases was 91.71% and above. Compared with the control, 596 differentially expressed genes were significantly up-regulated and 1 025 genes were down-regulated in drought treatment group. Compared with drought treatment group, 394 differentially expressed genes were significantly up-regulated and 117 genes were down-regulated in drought+ABA treatment group. GO enrichment analysis showed that the enriched category was metabolic processes related genes in the three treated samples. At the aspects of cellular components and molecular functions, the most enriched category was cell part and catalytic activity. KEGG enrichment analysis illustrated that 260 differentially expressed genes involved in 72 pathways in drought treatment group sample. There were 66 differentially expressed genes involved in 38 metabolic pathways in drought+ABA treatment group sample compared with drought treatment group sample. Some genes were screened via KEGG significance enrichment analysis, which included plant hormone signal transduction, starch and sucrose metabolism. Finally, the results of transcriptome analyses were verified by real-time quantitative PCR.更多还原