【摘要】 为了解观赏禽元宝鸡中禽白血病病毒(ALV)的流行特点,通过接种DF-1细胞、ELISA和PCR检测,从元宝鸡中分离并鉴定出1株禽白血病病毒,命名为BJ1401。经PCR扩增、测序获得BJ1401的全基因序列。序列比对分析发现,病毒株BJ1401基因全长7 503 bp,其中,gp85基因核苷酸序列与C亚群同源性最高(91.6%),gp37、LTR与E亚群相应核苷酸序列同源性最高,分别为98.2%、91.6%。进化分析显示,gp85与C亚群代表株Prague C亲缘关系最近,gp37、LTR与E亚群代表株ev-1和SD0501处在同一进化分支上。表明观赏性元宝鸡中分离的禽白血病病毒BJ1401可能是C亚型与E型禽白血病病毒的重组病毒。更多还原

【Abstract】 In order to investigate the gene characteristics of avian leukosis virus in ornamental chickens, one avian leukosis virus strain, named as BJ1401, was isolated and identified via DF1 cell culture, ELISA and PCR assay. One whole genome of ALV strain was amplified,cloned and sequenced from the genomic DNA of DF1+. Results showed that the full length cDNA of the BJ1401 strain was 7 503 bp.The gp85 nucleotide sequence in the isolate displayed a high identity(91.6%) with that of the gp85 gene in ALV-C. The gp37 and LTR nucleotide sequences had the highest similarities with those of ALV-E. Phylogenetic analysis of the nucleotide sequences showed that the gp85 nucleotide sequence in the isolate displayed a high genetic relationship with ALV-C representative strain Prague C. gp37 and LTR were in the same evolutionary branches with representative endogenous ALV strains ev1 and SD0501. The above results show that the isolate is a recombinant between ALV-C and ALV-E.更多还原