【摘要】 为了提高双歧杆菌的抗逆性,该试验建立基于非水溶性膳食纤维的成膜体系,并优化了双歧杆菌生物膜成膜条件。以大豆纤维(添加量10 g/L)为成膜基质,以成膜活菌数为响应值,通过单因素试验研究碳源、氮源、培养温度、初始pH值以及NaCl添加量对双歧杆菌成膜的影响。结果表明,最显著的影响因素为初始p H值,培养温度和NaCl添加量。Box-Behnken响应曲面分析得到最优的成膜条件为:培养基碳源为葡萄糖、氮源为胰蛋白胨、额外添加NaCl 0.35%、培养温度37℃,初始pH值7.0。在此最优条件下制备的双歧杆菌成膜活菌数最多为1.22×10~9 CFU/m L,为实现双歧杆菌生物膜的可控成膜,特别是工业规模的发酵制备提供了理论基础和实验依据。更多还原

【Abstract】 In order to improve the resistance of Bifidobacterium, a biofilm forming system based on insoluble dietary fiber was established, and the conditions for Bifidobacterium biofilm formation were optimized. Using soybean fiber(addition 10 g/L) as the biofilm-forming substrate, and the number of viable bacteria as the response value, the effect of carbon source, nitrogen source, culture temperature, initial p H and Na Cl addition on Bifidobacterium biofilm formation was investigated by single factor experiments. The results showed that the most significant factors were initial p H, culture temperature and Na Cl addition. The Box-Behnken response surface experiments results showed that the optimum biofilm forming conditions were as follows: glucose as carbon source, tryptone as nitrogen source, Na Cl addition 0.35%, culture temperature 37 ℃, initial p H 7.0. The number of viable bacteria in the biofilm formation of Bifidobacterium prepared under the optimal conditions was 1.22×109 CFU/ml, and the number of viable bacteria was the highest. It provided a theoretical and experimental basis for the realization of controllable biofilm formation of Bifidobacterium, especially for the fermentation and preparation of Bifidobacterium biofilm on an industrial scale.更多还原