【摘要】 目的:探究circ₀001429通过调控miR-139-5p/转录生长因子影响因子1（TGF-interacting factor 1,TGIF1）分子轴对膀胱癌T24细胞恶性生物学行为的影响。方法:采用q PCR实验检测circ₀001429在膀胱癌细胞系SW780、T24、5637和人膀胱上皮永生化细胞SV-HUC-1中的表达水平,双荧光素酶报告基因实验验证miR-139-5p、circ₀001429与TGIF1之间的靶向调控关系;将T24细胞分为NC组、sh-circ₀001429组、miR-139-5p mimics组、sh-TGIF1组、pcDNA-circ₀001429+sh-TGIF1组、miR-139-5p mimics+pcDNA-TGIF1组以及sh-circ₀001429+miR-139-5p inhibitor组,Western blotting检测各组细胞中TGIF1的表达水平,CCK-8法、Transwell实验和流式细胞术分别检测circ₀001429、miR-139-5p和TGIF1对T24细胞增殖、侵袭、迁移和凋亡的影响。结果:circ₀001429在3珠膀胱癌细胞系中呈高表达（均P<0.01）,敲降circ₀001429可以显著抑制T24细胞增殖、侵袭、迁移并促进细胞凋亡（P<0.05或P<0.01）;双荧光素酶报告基因验证结果显示,circ₀001429与miR-139-5p、miR-139-5p与TGIF1存在靶向关系;过表达miR-139-5p显著抑制T24细胞增殖、侵袭、迁移并促进细胞凋亡（均P<0.01）。回复实验进一步证实circ₀001429和TGIF1竞争性结合miR-139-5p促进T24细胞增殖、侵袭、迁移且抑制细胞凋亡（均P<0.01）。结论:circ₀001429与TGIF1竞争结合miR-139-5p促进膀胱癌细胞T24的增殖、侵袭、迁移且抑制细胞凋亡。更多还原

【Abstract】 Objective: To explore the effect of circ₀001429 on proliferation and apoptosis of bladder cancer cells by regulating miR-139-5 p/TGF-interacting factor 1（TGIF1）axis. Methods: The expression of circ₀001429 in bladder cancer cell lines SW780, T24,5637 and human bladder epithelial SV-HUC-1 cells were detected by RT-qPCR. Targeted regulatory relationship between circ₀001429 and miR-139-5 p as well as miR-139-5 p and TGIF1 was measured by Dual luciferase reporter gene assay. T24 cells were divided into NC group, sh-circ₀001429 group, miR-139-5 p mimics group, sh-TGIF1 group, pcDNA-circ₀001429+sh-TGIF1 group, miR-139-5 p mimics+pcDNA-TGIF1 group and sh-circ₀001429+miR-139-5 p inhibitor group. Western blotting was used to detect the expression level of TGIF1 in each group. CCK-8 method, Transwell experiment and Flow cytometry were used to detect the effects of circ₀001429, miR-139-5 p and TGIF1 on proliferation, invasion, migration and apoptosis of T24 cells, respectively. Results: Circ₀001429 was highly expressed in three bladder cancer cell lines（P<0.01）. Knockdown of circ₀001429 significantly inhibited proliferation,invasion and migration of T24 cells while promoted the level of cell apoptosis（P<0.05 or P<0.01）. The results of Dual luciferase reporter gene assay confirmed that there is a targeting relationship between circ₀001429 and miR-139-5 p as well as between miR-139-5 p and TGIF1. Overexpression of miR-139-5 p significantly inhibited the proliferation, invasion and migration of T24 cells while promoted the level of cell apoptosis（all P<0.01）. Recovery experiments further confirmed that the competitive binding of circ₀001429 and TGIF1 to miR-139-5 p promoted the proliferation, invasion and migration of T24 cells while inhibited the level of cell apoptosis（all P<0.01）. Conclusion: Circ₀001429 promotes proliferation, invasion and migration and inhibits apoptosis of bladder cancer T24 cells by competing with TGIF1 to bind to miR-139-5 p.更多还原