【摘要】 目的观察高效液相色谱-二极管阵列检测器（HPLC-DAD）检测人血浆中美罗培南浓度的效果。方法采用HPLC-DAD检测人血浆中美罗培南浓度,以雷尼替丁为内标物,以甲醇为蛋白沉淀剂进行前处理去除血浆蛋白,使用Agilent C18（4.6 mm×100 mm,3.6μm）色谱柱分离,同时去除内源性和外源性物质干扰,流动相25 mmol/L KH₂PO₄溶液-乙腈（92∶8,V/V）,流速0.8 ml/min,进样量50μl,检测波长和参比波长分别是308、380 nm。观察其专属性、标准曲线、精密度、回收率和稳定性。结果美罗培南在0.515～103.1μg/ml内线性关系良好（r=0.99978）,定量下限为0.515μg/ml。美罗培南在低、中、高浓度质控（2.06、20.6和82.5μg/ml）方法回收率在94.21%～107.61%,日内和日间的精密度相对标准偏差均<4%。结论 HPLC-DAD法灵敏度高、专属性强、定量准确,适用于人血浆中美罗培南的药物浓度监测。更多还原

【Abstract】 Objective To establish a high performance liquid chromatography-diode array detector(HPLC-DAD method to determine meropenem in human plasma samples. Methods Ranitidine was used as the internal standard. The samples were pretreated with methanol to precipitate and remove plasma proteins. The analytes were separated on the Agilent C18 column（4.6 mm×100 mm, 3.6 μm）, and the interference of endogenous and exogenous substances were removed with a mobile phase consisting of 25 mmol/L KH₂PO₄-acetonitrile（92∶8, V/V, pH 5.5）. The flow rate was 0.8 ml/min, the injection volume was 50 μl, the detective wavelength and reference wavelength were 308 nm and 380 nm, respectively. The specificity, standard curve, precision, recovery rate and stability of the method were investigated. Results The liner range of meropenem in human plasma samples was 0.515-103.0 μg/ml（r=0.99978）. The limit of quantitation was 0.515 μg/ml. The low, medium and high quality controls（2.06, 20.6 and 82.5 μg/ml） of extraction recoveries ranged from 94.21% to 107.61%, the RSD of intra-day and inter-day assay were lower than 4%. Conclusion The established HPLC-DAD method is simple and accurate for the determination of the concentration of meropenem in human plasma samples.更多还原