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不同方法破除黄花苜蓿新品系种子硬实的效果研究
Study on the Effects of Different Treatments on Breaking the Hard-seededness of New Medicago falcata Variety
【摘要】 为获得破除黄花苜蓿新品系种子硬实特性的最佳方法,采用低温,液氮,热水浸种,高温干燥,砂纸打磨,激素(赤霉素GA),酸(HCl、H2SO4)、碱(NaOH)、盐(KMnO4、KNO3、NaCl)和双氧水(H2O2)13种处理方法,对破除黄花苜蓿新品系种子硬实的效果开展研究。结果表明:适度砂纸打磨处理效果最好,能完全破除黄花苜蓿的种子硬实,发芽率可达98%;其次是98%浓硫酸处理种子25~60 min,硬实率可下降到16%以下,即发芽率可达84%以上;再次是100℃热水处理,能使发芽率提高到62%,硬实率降至38%。3~15 min的液氮处理,也能使发芽率达62%~64%,但幼苗在后期生长过程中死亡率高达20%~26%,不宜采用。其它处理,如70℃高温干燥0.5~3 h处理,-18℃7 d的处理等,与对照相比效果显著(p<0.05),但处理后的种子硬实率依然在45%以上,在生产中的实际应用价值较小。KNO3的所有处理对破除参试黄花苜蓿种子硬实的效果均不显著。
【Abstract】 In order to obtain the best way of breaking the hard-seededness of new Medicago falcata variety,13 treatment methods including low temperature,liquid nitrogen quick freezing,seed soaking with hot water,high temperature drying,sanding,GA3 treatment,HCl/H2SO4 treatment,NaOH treatment,KMnO4/KNO3/NaCl treatment and H2O2 treatment.The results showed that moderate sanding treatment was the best which could completely break the hard-seededness of M. falcata and the germination rate could reach 98%,followed by the treatment with 98% concentrated sulfuric acid for 25-60 min and hard-seededness rate could be reduced to less than 16%,that was,germination rate could reach more than 84%.Finally,the treatment with 100 ℃ hot water which could increase the germination rate to 62% and decrease hard-seededness rate to 38%.In addition,liquid nitrogen treatment for 3-15 min could also increase the germination rate to 62%-64%,but the seedling mortality was as high as 20%-26% in the late growth process,so it was not suitable to be used.Other treatments, such as drying at 70 ℃ for 0.5-3 h and drying at-18 ℃ for 7 d showed significant influence compared with the control group(p<0.05).However,the hard-seededness rate was still above 45% after treatment,which had little practical application value in production.And,KNO3 treatments had no significant effect on breaking the hard-seededness of M. falcata.
- 【文献出处】 种子 ,Seed , 编辑部邮箱 ,2020年06期
- 【分类号】S541.9
- 【被引频次】13
- 【下载频次】290