【摘要】 目的探讨CD40基因rs1883832多态性与老年性骨质疏松性骨折患者骨代谢标志物和骨密度的关系。方法研究对象为183例老年骨质疏松性骨折患者(骨折组)和163例老年骨质疏松患者(对照组)。采用SNa Pshot法进行SNP分型,化学发光法测定骨代谢标志物PINP和β-CTX水平,双能X线吸收仪测定腰椎L2-4和髋部骨密度。分析两组等位基因频率和基因型频率有无差异,并分析两组rs1883832基因多态性与骨代谢标志物和各部位骨密度的关系。结果对照组基因型频率符合哈迪温伯格平衡(P>0. 05)。骨折组和对照组A、G等位基因频率分别为30. 3%、69. 7%和32. 2%、67. 8%(P>0. 05);两组AA、AG和GG基因型频率分别为11. 5%、37. 7%、50. 8%和10. 4%、43. 6%、46. 0%(P>0. 05)。骨折组骨转换标志物水平高于对照组而各部位骨密度低于对照组;两组AA基因型各部位骨密度均低于AG、GG基因型(P>0. 05)。结论未发现CD40基因rs1883832多态性与老年骨质疏松性骨折患者骨代谢标志物和骨密度存在相关性。更多还原

【Abstract】 Objective To investigate the relationship between CD40 gene rs1883832 polymorphism and bone metabolism markers and bone mineral density in elderly patients with osteoporotic fractures. Methods One hundred and eighty-three elderly patients with osteoporotic fractures( fracture group) and 163 elderly patients with osteoporosis( control group) were enrolled in the study.SNP identification was performed using SNaPshot technology. Concentrations of metabolism markers PINP and β-CTX were determined using chemiluminescence method. The bone mineral density of lumbar vertebrae L2-4 and hip was measured using dual energy X-ray absorptiometry. The differences in allele frequencies and genotype frequencies between the two groups were analyzed.The relationship between rs1883832 polymorphism and bone metabolism markers and bone mineral density was explored. Results The genotype frequency of the control group was consistent with Hardy Weinberg equilibrium( P > 0. 05). The A and G allele frequencies in the fracture group and the control group were 30. 3% and 69. 7%,and 32. 2% and 67. 8%,respectively( P>0. 05).The frequencies of AA,AG,and GG genotypes in the two groups were 11. 5%,37. 7%,50. 8%,and 10. 4%,43. 6%,46. 0%,respectively( P>0. 05). The bone turnover markers in the fracture group were higher than that in the control group,and the bone mineral density in each site was lower than that in the control group. The bone mineral density in each group of AA genotypes was lower than that of AG and GG genotypes( P>0. 05). Conclusion The relationship between the rs1883832 polymorphism of CD40 gene and bone metabolism markers and bone mineral density in elderly patients with osteoporotic fractures was not detected in our study.更多还原