【摘要】 为了获得具有溶磷、抑菌作用的根际促生菌,分析其对烤烟的防病、促生效果,从潍坊烟区根际土壤中筛选出溶磷细菌,并进行recA基因鉴定,通过室内、田间试验研究其抑菌和促生效果。结果表明,从土壤中筛选到10株溶磷效果较好的菌株,其中菌株CT45-1溶磷、抑菌、促生效果较好,经鉴定为新洋葱伯克霍尔德氏菌(Burkholderiacenocepacia);选用此菌株在温室、田间试验中单独及与草酸青霉QM-10混合施用,均能提高烤烟对磷的吸收利用,改善烤烟农艺性状,提高烟叶产量和品质,并在一定程度上提高烟株抗病能力。其中以二者混合施用效果更好,可进一步推广应用。更多还原

【Abstract】 In order to obtain the rhizosphere growth-promoting bacteria with the function of phosphorus solubilization and bacteriostasis, the bacteria strains were isolated from the tobacco rhizosphere soil in Weifang, and identified by recA genes sequencing analysis. The bacteriostasis and growth promoting effect of the bacteria on tobacco were studied with pot and field experiments. The results showed that 10 strains of bacteria with phosphorus solubilizing ability were isolated from the soil. The strain CT45-1, which was identified as Burkholderia cenocepaci showed the best phosphate solubilizing, bacteriostatic and growth promoting effects. The pot and field experiments showed that, the application of CT45-1 individually or mixed with Penicillium oxalate QM-10 could improve the utilization efficiency of phosphorus, improve the yield and quality of flue cured tobacco, and improve the disease resistance of tobacco to a certain extent. The mixed application of the two strains showed better effects, which could be further popularized and applied.更多还原