【摘要】 为探究免疫增强剂CVC1302对O型口蹄疫(FMD)细菌样颗粒(BLP)疫苗的免疫增强作用,本研究将革兰氏阳性增强基质(GEM)处理的口蹄疫病毒(FMDV)VP1结构蛋白表位的重组蛋白B(T1BT2)4B与CVC1302混合,与白油佐剂乳化制成疫苗,免疫4周龄ICR小鼠及45日龄FMDV抗体阴性仔猪。免疫后利用FMDV VP1结构蛋白ELISA抗体检测试剂盒检测小鼠血清中的特异性ELISA抗体水平;利用淋巴细胞增殖试验检测免疫小鼠淋巴细胞增殖情况;利用荧光定量PCR方法检测免疫小鼠相关细胞因子水平以及利用液相阻断抗体检测试剂盒检测免疫仔猪血清中的液相阻断抗体滴度。结果显示,免疫增强剂CVC1302可以显著提高GEM-B(T1BT2)4B免疫后小鼠血清中的抗体水平(p<0.05);小鼠T淋巴细胞增殖率及细胞因子IL-4、IFN-γ的转录水平均显著升高(p<0.05);仔猪血清中的液相阻断抗体水平也明显高于对照组。以上结果表明免疫增强剂CVC1302可以提高O型FMD BLP疫苗的细胞免疫和体液免疫水平,对FMD BLP疫苗具有较好的免疫增强作用。本研究为提高FMD亚单位疫苗的免疫效力提供了新的思路。更多还原

【Abstract】 To evaluate the efficacy of immunopotentiator CVC1302 on bacterial-like particle(BLP) vaccine against type O foot-and-mouth disease virus(FMDV), the recombinant protein B(T1 BT2)4 B displayed on Gram-positive enhancer matrix was mixed with or without CVC1302 before emulsified with oil adjuvant. Four-week-old female mice and 45-day-old FMDV negative piglets were immunized with the vaccine, respectively. In mice, we detected the FMDV VP1-specific antibody titers, the level of lymphocytes proliferation and the mRNA expression levels of IL-4 and IFN-γ to evaluate the adjuvanticity of CVC1302. In pigs, the antibody titers against FMDV were detected by liquid-phase blocking(LBP) ELISA. The results of mice experiments showed that CVC1302 significantly improved the production of FMDV-specific antibodies, the levels of lymphocytes proliferation and the expressions of Th1 or Th2 type cytokines induced by GEM-B(T1 BT2)4 B. Results of the piglets study revealed that CVC1302 played an important role in inducing higher titers of LBP antibodies against FMDV in group inoculated with CVC1302-adjuvanted vaccine when compared with the group inoculated with vaccine without CVC1302. In conclusion, CVC1302 is a good immunopotentiator for FMDV bacterial like particle vaccine and can improve the cellular and humoral immune responses induced by the BLP vaccine. This study provided a new approach to improve the efficacy of FMDV subunit vaccines.更多还原