【摘要】 目的:观察甘麦大枣汤对抑郁模型大鼠行为学及单胺递质的影响,并从突触结构及结构蛋白MAP-2与GAP-43表达改变探讨其潜在作用机制。方法:60只SD大鼠随机分为5组:正常对照组、模型组、氟西汀组(10.8mg/kg)、甘麦大枣汤高、低剂量组(9.72、4.86 g/kg),每组12只。除对照组外,其余各组大鼠均采用慢性不可预见性温和应激(CUMS)建立抑郁模型,并于造模同时给药组灌胃给药,连续21 d。采用糖水消耗实验和旷场测试评价大鼠抑郁样行为,ELISA法检测海马单胺递质5-HT、NE含量,Golgi染色观察神经元突触损伤情况,免疫组化法和Western blot法检测海马突触结构蛋白MAP-2和GAP-43的表达。结果:与对照组比较,抑郁模型大鼠糖水偏好度及自主活动评分均显著下降(P<0.01),海马5-HT、NE含量显著下降(P<0.01),树突棘缺失明显,同时MAP-2和GAP-43表达均显著下调(P<0.01);甘麦大枣汤干预后,模型大鼠抑郁样行为明显缓解(P<0.01),5-HT、NE含量显著升高(P<0.05),树突棘密度、长度及分枝增加,MAP-2和GAP-43表达显著回升(P<0.01)。结论:甘麦大枣汤能改善抑郁模型大鼠的抑郁样行为,升高海马单胺递质含量,其作用可能与上调突触结构蛋白、缓解神经元突触损伤有关。更多还原

【Abstract】 Objective: To investigate the effects of Ganmai Dazao Tang on behavior and monoamine neurotransmitters in rats with depression,and to explore its potential mechanism from synaptic structure.Methods: Sixty SD rats were randomly divided into 5 groups: normal control group,model group,fluoxetine group( 10.8 mg/kg),Ganmai Dazao Tang high and low dose group( 9.72,4.86 g/kg),12 rats in each group.Except the control group,the rats in the other groups were all chronically unpredictable mild stress( CUMS) to establish a depression model,and were treated by intragastric administration for 21 days.The depression-like behaviors of rats were evaluated by sucrose consumption test and open field test.The contents of serotonin( 5-HT) and norepinephrine( NE) in hippocampus were detected by ELISA.The synaptic damage of neurons was observed by Golgi staining.The synaptic structure protein expression levels of MAP-2 and GAP-43 of hippocampus were detected by Immunohistochemistry and Western blot.Results: Compared with control group,the sucrose preference and autonomic activity scores of the depression model rats were decreased significantly( P<0.01),the levels of 5-HT and NE in hippocampus were decreased significantly( P<0.01),and the dendritic spines were absent,and the expressions of MAP-2 and GAP-43 were down-regulated significantly( P< 0.01).After treated with Ganmai Dazao Tang,the depression-like behavior of the model rats was significantly relieved( P < 0.01),and the levels of 5-HT and NE were increased( P <0.05).Dendritic spine density,length and branching were increased,the expressions of MAP-2 and GAP-43 were increased( P <0.01).Conclusion: Ganmai Dazao Tang can improve the depression-like behavior of depression model rats and increase the monoamine neurotransmitter content in hippocampus,which may be related to up-regulation of synaptic structural proteins and relief of synaptic damage in neurons.更多还原