【摘要】 目的探讨新诊断胶质瘤O6-甲基鸟嘌呤-DNA甲基转移酶(MGMT)基因启动子甲基化(简称MGMT甲基化)水平的变化。方法回顾性分析2016年1月至2019年6月郑州大学附属肿瘤医院神经外科收治的225例新诊断胶质瘤的临床资料,定量分析MGMT甲基化。结果新诊断胶质瘤MGMT甲基化水平为(23.68±14.88)%,阳性率89.33%。≥40岁病人MGMT甲基化水平[(24.89±15.67)%]显著高于<40岁病人[(19.85±11.31)%;P<0.05]。少突胶质细胞瘤MGMT甲基化水平[(30.41±12.99)%]显著高于其他类型的胶质瘤[星形细胞瘤为(20.81±13.53)%、少突星形细胞瘤为(23.00±8.21)%、胶质母细胞瘤为(23.39±17.85)%;P<0.05]。胶质瘤MGMT甲基化水平与病人年龄呈正相关(r=0.135,P<0.05),而与病人性别和肿瘤级别无明显关系(P>0.05)。结论新诊断胶质瘤MGMT甲基化水平与病人与年龄正相关,少突胶质细胞瘤MGMT甲基化水平明显高于其他类型胶质瘤。更多还原

【Abstract】 Objective To explore the change of methylation level of O6-methylguanine-DNA methyltransferase(MGMT) gene promoter in newly diagnosed glioma. Methods The methylation level of MGMT gene promoter of 225 newly diagnosed glioma patients who were admitted to our hospital from January 2016 to June 2019 were analyzed retrospectively. Results The mean methylation level of MGMT gene promoter in newly diagnosed glioma patients was(23.68+14.88)% with a positive rate of 89.33%. The methylation level in patients over 40 years [(24.89+15.67)%] was significantly higher than that [(19.85 +11.31)%] under 40 years(P<0.05). The methylation level was significantly higher in patients with oligodendroglioma [(30.41+12.99)%] than patients with astrocytoma [(20.81+13.53)%, P<0.05], oligoastrocytoma [(23.00±8.21)%, P<0.05], or glioblastoma [(23.39±17.85)%, P<0.05]. There were no significant differences in the methylation level between different gender and grade of tumour(P>0.05). The methylation level of MGMT was positively related to the age(r=0.135, P<0.05), but not related to the gender and the grade of glioma(P>0.05). Conclusions The methylation level of MGMT gene promoter is not related to the gender and the grade of glioma, but positively related to the age. The methylation level of MGMT of oligodendroglioma is higher than other glioma types.更多还原