【摘要】 为深入了解严重急性呼吸综合征冠状病毒2型(SARS-CoV-2)S、M、E、N蛋白的分子生物学特征和基本结构特征,选取S、M、E、N蛋白序列进行生物信息学分析和预测,使用DNAstar、SignalP、TMHHM、PSORT Prediction、BUSCA和ABCpred软件,分析并相互验证4种蛋白的结构域特征和S蛋白的潜在B细胞抗原表位,结果预测出了4种结构蛋白的功能结构域,并分析预测出S蛋白潜在的B细胞抗原表位为25～29、75～81、112～116、148～152、773～779 aa。研究结果可为SARS-CoV-2相关的分子生物学和结构生物学研究提供参考,并为疫苗开发等提供理论依据。更多还原

【Abstract】 In order to further identify the molecular biological characteristics and general structural characteristics of S,M,E and N proteins of severe acute respiratory syndrome coronavirus type 2(SARS-CoV-2),the amino acid sequences of the above proteins were selected for bioinformatics analysis and prediction. The structural domain characteristics of the above proteins and the potential B cell epitopes of S protein were analyzed and mutually verified by DNAstar,SignalP,TMHHM,PSORT Prediction,BUSCA and ABCpred softwares. The results showed that the functional domains of the four proteins were predicted,and the potential B cell epitopes of S protein was analyzed and predicted to be 25 to 29,75 to 81,112 to 116,148 to 152 and 773 to 779 aa. Based on the results,some references were provided for further study on molecular biology and structural biology related to SARS-CoV-2,and theoretical basis was provided for vaccine development.更多还原