【摘要】 目的:观察芒柄花黄素对卵巢癌细胞增殖、迁移与侵袭的影响,并初步探讨其机制。方法:体外培养人卵巢浆液性囊腺癌SKOV-3细胞,采用不同浓度(0、25、50和100μmol/L)芒柄花黄素处理细胞48 h,用MTS法检测细胞活力,并筛选出半数抑制浓度。通过划痕迁移实验和Transwell侵袭实验检测芒柄花黄素对SKOV-3细胞迁移与侵袭能力的影响。RT-qPCR法和Western blot法检测细胞中的上皮钙黏素(E-cadherin)和基质金属蛋白酶9(MMP-9)mRNA及蛋白表达的变化。结果:与对照组比较,SKOV-3细胞的活力随着芒柄花黄素浓度增加而下降(P<0.01)。50μmol/L芒柄花黄素组细胞迁移距离少于对照组(P<0.01)。50μmol/L芒柄花黄素组侵袭穿过Transwell小室底膜的细胞数量显著减少(P<0.01)。此外,50μmol/L芒柄花黄素组E-cadherin的mRNA及蛋白水平显著高于对照组(P<0.01),而MMP-9的mRNA及蛋白水平显著低于对照组(P<0.01)。结论:芒柄花黄素可能通过增加E-cadherin和降低MMP-9的表达来抑制卵巢癌SKOV-3细胞的迁移和侵袭。更多还原

【Abstract】 AIM:To observe the effect of formononetin on the viability,migration and invasion of ovarian cancer cells,and to explore its mechanism.METHODS:Human ovarian serous cystadenocarcinoma SKOV-3 cells were cultured in vitro. The cells were treated with formononetin at 0,25,50 and 100 μmol/L for 48 h. The cell viability was measured by MTS assay. The migration and invasion abilities of the SKOV-3 cells were detected by scratch wound assay and Transwell assay. RT-qPCR and Western blot were used to detect the mRNA and protein levels of E-cadherin and matrix metalloproteinase-9(MMP-9).RESULTS:The viability of SKOV-3 cells was decreased with the increase in the formononetin concentration compared with control group(P<0. 01). The wound migration distance of the cells in 50 μmol/L formononetin group was less than that in control group(P<0. 01). The number of invasive SKOV-3 cells across the Transwell sub-compartment was significantly decreased in 50 μmol/L formononetin group compared with control group(P<0. 01). The mRNA and protein levels of E-cadherin in 50 μmol/L formononetin group were significantly higher than those in control group(P<0. 01),while the mRNA and protein levels of MMP-9 in 50 μmol/L formononetin group were significantly lower than those in control group(P<0. 01).CONCLUSION:Formononetin inhibits the migration and invasion abilities of ovarian cancer SKOV-3 cells by increasing expression of E-cadherin and decreasing expression of MMP-9.更多还原