【摘要】 目的建立同时测定乌蕨中原儿茶酸、原儿茶醛、荭草苷、牡荆素鼠李糖苷、牡荆素、木犀草苷等6种主要活性成分含量的双波长高效液相色谱法。方法选用赛默飞（Thermo）A型C₁₈柱（4.6 mm×250 mm,5μm）,以乙腈（A）-0.4%磷酸溶液（B）为流动相,梯度洗脱,原儿茶酸、原儿茶醛检测波长为280 nm,荭草苷、牡荆素鼠李糖苷、牡荆素、木犀草苷检测波长为350 nm。柱温:30℃,流速:1 mL·min^-1;进样量:5μL。结果乌蕨中原儿茶酸、原儿茶醛、荭草苷、牡荆素鼠李糖苷、牡荆素、木犀草苷六种活性成分分离度良好,质量分别在0.161 9～1.214 4,0.063 7～0.477 9,0.083 5～0.626 5,0.238 5～1.788 5,0.077 8～0.583 7,0.026 3～0.197 0μg呈良好的线性关系,均r≥0.999 9,平均回收率分别为100.42%,101.78%,99.17%,105.12%,97.86%,101.54%;RSD分别为2.38%,2.53%,2.31%,3.06%,2.13%,3.87%。结论该方法简单,结果准确,重复性、稳定性好,可为制订乌蕨的质量控制标准提供参考依据。更多还原

【Abstract】 Objective A dual wavelength high performance liquid chromatography（HPLC） method was established for the simultaneous determination of protocatechuic acid, protocatechuic aldehyde, orientin, oramnosylvitexin, vitexin, and luteolin-7-o-glucoside in Stenoloma chusanum（L.） Ching. Methods The analysis was performed on a Thermo-Finnegan C₁₈（250 mm×4.6 mm,5 μm） column with mobile phase of acetonitrile（A）-0.4% phosphoric acid solution（B） at the flow rate of 1.0 mL·min^-1for gradient elution.The determintion wavelength was 280 nm for protocatechuic acid and protocatechui caldehyde, and 350 nm for orientin, oramnosylvitexin, vitexin and luteolin-7-o-glucoside.The column temperature was 30 ℃ and injection volume was 5 μL. Results The six compounds were well separated. The linear ranges were from 0.161 9 to 1.214 4, 0.0637 to 0.477 9, 0.083 5 to 0.626 5, 0.238 5 to 1.788 5, 0.077 8 to 0.583 7, and 0.0263 to 0.197 0 μg for protocatechuic acid, protocatechuic aldehydeorientin, orientin, oramnosylvitexin, vitexin, luteolin-7-o-glucoside, respectively,with the correlation of r≥0.999 9. The average recoveries were 100.42%（RSD 2.38%）, 101.78%（RSD 2.53%）, 99.17%（RSD 2.31%）, 105.12%（RSD 3.06%）, 97.86%（RSD 2.13%） and 101.54%（RSD 3.87%）. Conclusion The method is simple, accurate, reproducible and stable, which can provide reference for the quality control standard of Stenoloma chusanum（L.）Ching.更多还原