【摘要】 目的探讨大连石城岛原驻老年居民高血压的患病率及相关因素,血清对氧磷酶1 (rs662)基因(PON1rs662)与高血压的关系。方法选取2016年6—8月石城岛654名65岁以上原驻居民对其进行高血压及相关因素流行病学调查,分别采用调整的世界卫生组织(WHO)-MONICA研究问卷进行问卷调查,进行生化及心脏超声相关检查;采用离心柱法提取脱氧核糖核酸(DNA),实时定量荧光聚合酶链反应(PCR)技术(TaqManPCR)对PON1rs662进行基因分型,确定所有研究对象PON1rs662基因型分布是否符合哈温伯格平衡定律,探讨PON1rs662基因与高血压的关系。结果调查对象总人群高血压患病率为73.1%,女性患病率为77.5%,男性患病率为68.3%;高血压与非高血压人群心率、体重指数(BMI)、腰围、尿酸(UA)、三酰甘油(TG)、左房前后径(LA)比较,差异有统计学意义(P <0.05);研究对象PON1rs662基因型分布符合哈温伯格平衡(P> 0.05);等位基因模型和相加模型检测研究对象PON1rs662 SNP位点与高血压无相关性(P> 0.05)。结论大连石城岛原驻老年居民高血压患病率为73.1%;心率、BMI、腰围、UA、TG与岛上高血压患病率有关;PON1rs662基因与研究对象高血压患病率可能无相关性。更多还原

【Abstract】 Objective To investigate the prevalence and related factors of hypertension in the elderly in Shicheng Island of Dalian, and the relationship between serum P-oxophosphase 1(rs662) gene(PON1 rs662) and hypertension.Methods The epidemiological survey on hypertension and related factors was conducted among 654 residents aged more than 65 from June to August 2016. The adjusted WHO-MONICA research questionnaire was used for questionnaire survey, biochemical and echocardiographic examinations were carried out. DNA was extracted by centrifugation column, and gene typing of PON1 rs662 was carried out by TaqMan PCR. All the studies were determined whether the genotype distribution of PONIrs662 accord with Hardy-Weinberg Law, and the relationship between PON1 rs662 gene and hypertension was investigated. Results The prevalence of hypertension was 73.1%, 77.5% in women and 68.3% in men.There were statistically significant differences in heart rate, body mass index(BMI), waist circumference, uric acid(UA),triglyceride(TG) and left atrial anteropostery(LA) between hypertensive and non-hypertensive subjects(P < 0.05). The genotype distribution of PON1 rs662 was in line with Hardy-Weinberg Law(P > 0.05). Both the allele model and the additive moded showed no correlation between the PON1 rs662 SNP and hypertension(P > 0.05). Conclusion The prevalence rate of hypertension in the old residents of Shicheng Island was 73.1%. Heart rate, BMI, waist circumference, UA and TG were associated with the prevalence of hypertension on the island. PON1 rs662 gene may not be correlated with the prevalence of hypertension in these study objects.更多还原