【摘要】 目的研究急性髓细胞白血病(AML)患儿miR-126表达水平与预后的相关性。方法选取2014年5月至2016年12月本院收治的AML患儿58例为研究对象,纳入研究组(高危、中危、低危分别16、20、22例),均接受AML标准治疗,同时以本院同期入院的30例非恶性病儿童作为对照组,采用TagMan RT-PCR法检测2组骨髓标本中miR-126表达量,依据出院时miR-126表达量相对水平将AML患儿分为高表达组、低表达组各29例,比较2组miR-126表达量、中位生存时间、无事件生存时间、中位生存率,并根据1年内随访结果将其分为完全缓解组、难治复发组、死亡组,对比3组miR-126表达量,同时分析AML患儿miR-126表达水平与预后的关系。结果研究组miR-126表达量明显高于对照组(P<0.05),且研究组中miR-126表达量依次为高危患儿>中危患儿>低危患儿(P<0.05);miR-126高表达组miR-126表达量(1.52±0.27)高于低表达组,而其中位生存时间(6.21个月)、无事件生存时间(5.34个月)短于低表达组(8.24个月、7.12个月),中位生存率96.55%低于低表达组(P<0.05);完全缓解组出院时、末次随访时miR-126明显低于难治复发组、死亡组,难治复发组、死亡组miR-126表达水平差异也有统计学意义(P<0.05);相关性分析结果显示AML患儿miR-126表达水平与中位生存时间、无事件生存时间、中位生存率呈负相关(P<0.05)。结论 AML患儿中miR-126呈高表达状态,且miR-126表达水平与患儿预后关系密切,因而临床可结合miR-126基因检测以提高初治AML患儿的预测准确性。更多还原

【Abstract】 Objective To study the correlation between miR-126 expression and prognosis in children with acute myeloid leukemia(AML). Methods 58 children with AML who were admitted to the hospital during the period from May 2014 to December 2016 were included in the study group(16,20 and 22 cases in the high risk,moderate risk and low risk status). All of them were given standard treatment for AML. The30 children without malignant disease who were admitted to the hospital during the same period were enrolled as the control group. The expression quantities of miR-126 in bone marrow samples of the two groups were detected by TagMan RT-PCR method. Children with AML were divided into the high expression group and the low expression group according to the relative levels of miR-126 expression quantity at discharge,29 cases in each group. The miR-126 expression quantities,the median survival time,event-free survival time and median survival rate were compared between the two groups. The children were divided into the complete remission group,refractory recurrence group and death group according to the follow-up results within 1 year. The expression quantities of miR-126 were compared among the three groups. The relationship between miR-126 expression level and prognosis in children with AML was analyzed. Results The expression quantity of miR-126 in the study group was significantly higher than that in the control group(P<0.05). In the study group,the expression quantity of miR-126 was the highest in high-risk children,followed by moderate-risk children and low-risk children(P<0.05). The miR-126 expression quantity in the high expression group(1.52±0.27)was higher than that in the low expression group. The median survival time(6.21 months)and event-free survival time(5.34 months)were shorter than those in the low expression group(8.24 months,7.12 months),and the median survival rate(96.55%)was lower than that in the low expression group(P<0.05). The miR-126 in complete remission group was significantly lower than that in refractory recurrence group and death group at discharge and at the last follow-up(P<0.05). There were significant differences in miR-126 expression level between refractory recurrence group and death group(P<0.05). Correlation analysis showed that the expression level of miR-126 in children with AML was negatively correlated with the median survival time,eventfree survival time and median survival rate(P<0.05). Conclusions The miR-126 is highly expressed in AML children,and the expression level of miR-126 is closely related to the prognosis of children. Therefore,the miR-126 gene detection can be combined to improve the prediction accuracy for AML children of the initial treatment.更多还原