【摘要】 目的:探讨沉默信息调控因子(SIRT1)在N-甲基-D-天冬氨酸(NMDA)诱导下对肺泡上皮细胞损伤的保护作用。方法:将培养的小鼠肺上皮细胞MLE-12分为4组:Con组、NMDA组、NMDA+RSV组、RSV组。RSV为细胞SIRT1激动剂。处理24h后,CCK-8和LDH实验检测细胞活力和细胞毒性;试剂盒检测细胞培养液炎性因子TNF-α、IL-6水平;Western Blot检测细胞SIRT1表达水平。结果:与Con组相比,NMDA组细胞活力下降,培养液LDH和炎性因子水平增高,SIRT1蛋白表达降低(P<0.05)。与NMDA组相比,RSV干预使细胞活力增加,培养液LDH和炎性因子水平明显较低且SIRT1蛋白表达量增加(P<0.05)。结论:在NMDA诱导肺上皮细胞损伤中,激活SIRT1可以减轻NMDA诱导的炎性反应,具有细胞保护作用。更多还原

【Abstract】 Objective:To investigate the protective effect of SIRT1 on alveolar epithelial cell injury induced by NMDA.Methods:MLE-12 cells were divided into four groups:Con group, NMDA group, NMDA + RSV group and RSV group.RSV is a cell SIRT1 agonist.After 24 hours treatment,the cell viability and cytotoxicity were detected by CCK-8 and LDH experiments;the levels of TNF-α and IL-6 in the cell culture medium were detected by kit;the expression level of SIRT1 was detected by Western blot.Results:Compared with the Con group,the cell viability of NMDA group decreased,LDH and inflammatory factors in culture medium increased,and SIRT1 protein expression decreased(P<0.05).Compared with NMDA group,RSV intervention increased cell viability,significantly reduced LDH and inflammatory factors in culture medium,and increased SIRT1 protein expression(P<0.05).Conclusion:In NMDA induced lung epithelial cell injury,activation of SIRT1 can reduce the inflammatory response induced by NMDA,and has a cytoprotective effect.更多还原