【摘要】 目的探讨温阳通脉颗粒含药血清对氧化损伤的人脐静脉内皮细胞的保护作用,并探讨其可能的作用机制。方法将24只大鼠随机分为3组,每组8只,分别为空白血清组、低剂量含药血清组(0.125 g/mL)和高剂量血清组(0.25 g/mL)。体外培养人脐静脉内皮细胞,设立正常组、模型组(300μmol/L H2O2)、空白血清组(300μmol/L H2O2+10%空白血清)和温阳通脉低、高剂量组(300μmol/L H2O2+0.125 g/mL、0.25g/mL+10%含药血清)。MTT法测定细胞活力,生化法测超氧化物歧化酶活性和乳酸脱氢酶释放量,逆转录-聚合酶链反应检测内皮细胞人单核细胞趋化蛋白-1和人细胞间黏附分子-1 mRNA的表达。结果与模型组相比,温阳通脉颗粒组细胞活力和超氧化物歧化酶活性升高(P<0.01),乳酸脱氢酶释放降低(P<0.01),人细胞间黏附分子-1和人单核细胞趋化蛋白-1 mRNA表达明显下降(P<0.01)。结论温阳通脉颗粒含药血清对H2O2诱导的人脐静脉内皮细胞有保护作用,机制可能与其能减少炎症因子人细胞间黏附分子-1和人单核细胞趋化蛋白-1 mRNA的表达有关,能保护受损内皮细胞,延缓动脉粥样硬化等血管疾病的进程。更多还原

【Abstract】 Objective To explore the protective effect of Wenyang Tongmai Granule-containing serum against oxidative damage to human umbilical vein endothelial cells（HUVECs）, and explore the possible mechanism. Methods Twenty-four rats were randomly divided into the blank serum group, low-dose drug-containing serum group（0.125 g/mL） and high-dose serum group（0.25 g/mL） with 8 rats in each. HUVECs were cultured in vitro to establish a normal group, a model group（300 μmol/L H₂ O₂）, a blank serum group（300 μmol/LH2 O2+10% blank serum）, and low-dose and high-dose Wenyang Tongmai groups（300 μmol/L H₂O₂+ 0.125 g/mL, 0.25 g/mL, 10 % drug-containing serum）. MTT method was used to determine cell viability while the biochemical method was adopted to measure SOD activity and LDH release. MCP-1 and ICAM-1 mRNA expressions of endothelial cells were detected via RT-PCR. Results Compared with the model group, the cell viability and SOD activity of the Wenyang Tongmai groups increased（P<0.01）, LDH release decreased（P<0.01）, and ICAM-1 and MCP-1 mRNA expressions decreased significantly（P<0.01）. Conclusion Wenyang Tongmai Granule-containing serum has a protective effect on HUVECs induced by H2 O2. The mechanism may be related to its ability to reduce the expressions of inflammatory factors-ICAM-1 and MCP-1 mRNA. It can protect damaged endothelial cells and delay the progression of such vascular diseases as atherosclerosis.更多还原