【摘要】 目的:通过测定4种不同炮制工艺的附子及其煎煮液的主要生物碱的含量,以及4种炮制品的低、中、高三种浓度对阳虚小鼠免疫功能的影响,探讨附子4种不同炮制品的质量差异。方法:以6种主要生物碱含量为指标,通过HPLC法测定附子4种炮制品及其煎煮液的生物碱含量;考察4种炮制品煎煮液的不同浓度对阳虚小鼠免疫器官胸腺、脾脏脏器指数的影响。结果:附子4种炮制品高浓度组均能提高阳虚小鼠的体质量及增加脾脏、胸腺指数,与模型组相比具有统计学差异(P<0.05);同时高压炮制品组生物碱含量最高,小鼠的胸腺与脾脏指数最大。结论:附子高压炮制品煎煮液与传统黑顺片煎煮液相比,主要生物碱含量稍高,同时对免疫功能的提高作用稍强。更多还原

【Abstract】 Objective:To determine the content of main alkaloids of aconite and its decoction of 4 different processed products,and the effects of low,medium and high concentrations of 4 products on immunological function of Yang deficiency mice,to explore the quality differences of 4 processed aconite products.Methods:The 4 processed products and its decoction of the contents of six main alkaloids were determined by HPLC method.To investigate the effects of different concentrations of 4 processed aconite products decoction on thymus and spleen indexes of immune organs in mice with Yang deficiency.Results:The 4 processed aconite products with high concentrations could increase the body weight and spleen and thymus index of Yang deficiency mice,compared with model group was significantly different(P<0.05).Meanwhile,the content of alkaloids and the thymus and spleen indexes the high-pressure processed products group was the highest.Conclusion:Compared with traditional heishunpian,the content of main alkaloids and improving immunological function the high pressure processed products were better.更多还原