【摘要】 目的制备携hVEGF₁₆₅表达载体阳离子脂质微泡并测定其与hVEGF₁₆₅表达载体的结合率。方法制备携hVEGF₁₆₅表达载体阳离子脂质微泡,测定其粒径大小、浓度、Zeta电位;测定与hVEGF₁₆₅表达载体的结合率。结果阳离子脂质微泡90%粒径范围:1μm-3μm,平均粒径:（1.31±0.23）μm,平均浓度:（3.17±0.16）×10⁸/mL,Zeta电位:（28.00±1.84） mV,与hVEGF₁₆₅表达载体的最大结合率:（35.34±0.71）%。结论采用机械振荡法制备的阳离子脂质微泡,粒径分布良好,浓度、Zeta电位稳定,与hVEGF₁₆₅表达载体结合率较高,是携带基因表达的良好载体系统。更多还原

【Abstract】 Objective To prepare cationic lipid microvesicles carrying hVEGF₁₆₅ expression vector and determine its binding rate to hVEGF₁₆₅ expression vector. Methods Cationic lipid microbubbles containing hVEGF₁₆₅ expression vector were prepared and their particle size, concentration and Zeta potential were measured. The binding rate to hVEGF₁₆₅ expression vector was determined. Results The particle size range of 90% cationic lipid microbubbles was 1μm-3μm, the average particle size was（1.31±0.23）μm, the average concentration was（3.17±0.16）×10⁸/m L, the Zeta potential was（28.00±1.84）mV, and the maximum binding rate to hVEGF₁₆₅ expression vector was（35.34±0.71）%. Conclusion The cationic lipid microbubbles prepared by mechanical oscillation method have good particle size distribution, stable concentration and Zeta potential, and high binding rate with hVEGF₁₆₅ expression vector.更多还原