【摘要】 目的探讨肺栓塞患者T淋巴细胞分泌细胞因子基因表达。方法采用全人类基因组表达谱芯片,检测并分析20例肺栓塞患者(PE组)和20例非PE患者(对照组)T淋巴细胞分泌细胞因子基因表达差异。结果采用该芯片共检出T淋巴细胞分泌细胞因子相关基因14条。与对照组相比,PE组IL-2、IFN-γ、IL-17B、IL-23A和转化生长因子β3(TGF-β3) mRNA表达降低(P<0.05或P<0.01),而IL-10、TGF-β1 mRNA表达升高(P<0.01)。结论肺栓塞患者T淋巴细胞分泌细胞因子mRNA表达失衡,提示细胞免疫功能紊乱和/或缺陷,最终导致对病毒或胞内菌易感。更多还原

【Abstract】 Objective To investigate the expressions of cytokine genes secreted by T lymphocytes in the patients with pulmonary embolism(PE).Methods The whole human genome expression profile chip was used to detect and analyze the differences in gene expression of cytokines secreted by T lymphocytes in 20 patients with PE(group A) and 20 patient without PE as the controls(group C).Results There were 14 genes related to cytokine secretion by T lymphocytes detected by the gene chip.Compared with group C,the expressions of IL-2,IFN-γ,IL-17 B,IL-23 A and TGF-β3 mRNA were decreased(P<0.05 or P<0.01),while the expressions of IL-10 and TGF-β1 mRNA were increased in group A(P<0.01).Conclusion The expressions of cytokine mRNA secreted by T lymphocytes are imbalanced in the patients with PE,indicating a disorder and/or defect of cellular immune function,which ultimately leads to susceptibility to viruses or intracellular bacteria.更多还原