【摘要】 目的探讨卡泊三醇对人黑素细胞系PIG1增殖、迁移、酪氨酸酶活性及黑素含量的影响。方法 PIG1细胞分别采用卡泊三醇1×10^-10（A组）、1×10^-9（B组）、1×10^-8（C组）、1×10^-7（D组）和1×10^-6 mol/L（E组）处理;对照（F）组加入不含卡泊三醇的培养基。采用MTT法、微孔膜法、多巴氧化法及NaOH裂解法分别检测PIG1细胞的增殖、迁移、酪氨酸酶活性及黑素含量。结果C、D、E组PIG1细胞增殖率分别为（127.13±1.57）%、（136.17±1.40）%、（138.83±1.25）%,均高于F组的（100.00±2.05）%（P<0.05）。A组和B组PIG1细胞穿膜细胞数分别为（35.0±2.0）个和（46.3±6.0）个,多于F组的（24.0±2.7）个（P<0.05）。A、B、C、D组酪氨酸酶活性高于F组（0.386±0.005、0.408±0.009、0.425±0.011、0.361±0.005 vs.0.347±0.006）（P<0.05）。B组和C组黑素含量高于F组（0.106±0.004和0.125±0.005 vs.0.090±0.001）（P<0.05）。结论卡泊三醇可促进PIG1细胞的增殖、迁移,并可提高酪氨酸酶活性,从而促进其黑素合成。更多还原

【Abstract】 Objective To explore the effects of calcipotriol on the proliferation,migration, tyrosinase activity and melanin content of human melanocyte cell line PIG1.Methods The PIG1 cells were treated with calcipotriol 1×10^-10（group A）,1×10^-9（group B）,1×10^-8（group C）,1×10^-7（group D） and 1×10^-6 mol/L（group E）.Culture medium without calcipotriol was added in group F as the control.The proliferation,migration,tyrosinase activity and melanin content of PIG1 cells were detected with MTT,micropore filtration assay,DOPA oxidase assay and NaOH assay,respectively.Results Cell proliferation rate in groups of C,D and E was higher than that in group F [（127.13±1.57）%,（136.17±1.40）% and（138.83±1.25）% vs.（100.00±2.05）%]（P<0.05）.The number of PIG1 membrane-penetrating cells in groups of A and B was more than that in group F [（35.0±2.0） pieces and（46.3±6.0） pieces vs.（24.0±2.7） pieces]（P<0.05）.The activity of tyrosinase in groups of A,B,C and D was higher than that in group F（0.386±0.005,0.408±0.009,0.425±0.011 and 0.361±0.005 vs.0.347±0.006）（P<0.05）.Melanin content in groups of B and C was higher than that in group F（0.106±0.004 and 0.125±0.005 vs.0.090±0.001）（P<0.05）.Conclusion Calcipotriol can promote the proliferation and migration of PIG1 cells,improve the activity of tyrosinase,and promote its melanin synthesis.更多还原