【摘要】 【目的】探讨鱼鳞多肽亚铁螯合物的制备方法,为鱼鳞废弃物的利用提供新的途径和理论基础。【方法】以草鱼鱼鳞多肽为原料,氯化亚铁为铁源,通过优化螯合条件得到鱼鳞多肽亚铁螯合物;采用元素分析、紫外光谱、红外光谱、电镜扫描、X-射线粉末衍射分析技术对鱼鳞多肽和多肽亚铁螯合物的结构进行表征。【结果】选择抗坏血酸为抗氧化剂,抗氧化剂与氯化亚铁的质量比为0.15∶1,多肽与氯化亚铁质量比为2∶1,pH 5,反应时间40 min,反应温度30℃的条件下,亚铁螯合率为83.4%;红外光谱特征区-NH₂的吸收峰从3301.42 cm^-1移动至3362.61 cm^-1处,发生明显的位移;元素分析结果表明螯合物中含铁量为10.6%,N、C、H含量显著降低;扫描电镜观察到螯合后颗粒由圆球空心结构变为致密晶粒;X衍射图谱表明螯合物结晶度大幅提高。【结论】以草鱼鱼鳞和氯化亚铁为原料,按照上述条件得到的多肽亚铁螯合物产率高,亚铁离子与多肽配位明显。更多还原

【Abstract】 【Objective】In order to provide a new approach and theoretical basis for the utilization of fish scale waste,the preparation and structure change of fish scale polypeptide chelate iron was studied.【Method】The grass carp fish scale polypeptide and ferrous chloride were used as the raw material. The iron chelate was obtained by optimizing chelation conditions. The structure of fish scale polypeptide and iron-polypeptide chelates was characterized by elemental analysis, ultraviolet, infrared, electron microscope scanning and X diffraction analysis. 【Result】The antioxidant was ascorbic acid, the mass ratio of ascorbic acid to ferrite was 0.15∶1, the mass ratio of polypeptide to ferrous salt was 2∶1, pH value was 5, reaction time was 40 min and reaction temperature was 30 ℃. Under the optimal chelating conditions, the chelated rate was 83.4 %. The absorption peak of NH₂ in the characteristic region of infrared spectroscopy shifted from 3301.42 cm^-1 to 3362.61 cm^-1, resulting in obvious displacement. Elemental analysis showed that the iron content in the chelate was 10.625 %, and the content of N, C and H decreased significantly. Scanning electron microscopy showed that the chelated particles changed from spherical hollow structure to dense grains. X-ray diffraction patterns showed that the crystallinity of the chelate increased significantly. 【Conclusion】According to the above conditions, the chelation rate of peptide ferrous chelate is high, and the coordination between ferrous ion and peptide is obvious.更多还原