【摘要】 目的探讨自噬相关基因5（Atg5）在小鼠下腔静脉移植术后静脉桥血管狭窄过程中的作用。方法 30只雄性C57BL/6小鼠及20只Atg5^+/-小鼠分为WT假手术组（10只）,WT手术组（受体10只,供体10只）,Atg5^+/-手术组（受体10只,供体10只）。复制小鼠下腔静脉移植模型。HE染色观察下腔静脉管腔面积,Real-time PCR检测下腔静脉组织中Atg5、LC3 mRNA表达;Westem-Blot检测下腔静脉组织中Atg5、LC3蛋白表达,免疫荧光共染明确平滑肌细胞中LC3表达。结果 WT小鼠下腔静脉移植4周后出现管腔狭窄[（4.21±0.32）×10⁴μm²比（1.63±0.15）×10⁴μum²,P<0.05],Atg5[（0.51±0.17）×10^-3 比（1.49±0.08）×10^-3]、LC3[（1.9±0.4）×10^-2 比（3.8±0.9）×10^-2] mRNA表达均明显升高（均为P<0.05）。与WT小鼠相比,Atg5^+/-小鼠下腔静脉移植4周后Atg5[（0.39±0.05）比（0.16±0.08）]、LC3[（2.17±0.46）比（0.78±0.19）]蛋白表达均明显下降（均为P<0.05）,平滑肌细胞中LC3蛋白表达显著下降（P<0.05）,管腔狭窄明显减轻[（1.63±0.15）×10⁴μm²比（2.96±0.12）×10⁴μm²,P<0.05]。结论 Atg5下调抑制小鼠下腔静脉移植术后静脉桥血管狭窄。更多还原

【Abstract】 Objective To determinate the role of autophagy-related gene 5（Atg5） in the stenosis of vein graft after inferior vena cava transplantation in mice. Methods Thirty male C57 BL/6 wild type mice and 20 Atg5^+/- mice were divided randomly into into a WT sham operation group（n=10）, a WT surgery group（10 recipients, 10 donors）, and an Atg5^+/- surgery group（10 recipients, 10 donors）. HE staining was used to detect the stenosis of vein graft. The mRNA level of Atg5 and LC3 was detect by Real-time PCR. The protein of Atg5 and LC3 were measured by Westem-Blot. Immunofluorescence was used to analyze the specific cell type. Results The lumen of vein grafts in Atg5^+/- mice at 4 weeks after the inferior vena cava transplantation was wider than that in WT mice [（4.21±0.32）×10⁴ μm² vs.（1.63±0.15） ×10⁴ μm²,P<0.05], with increased Atg5 [（0.51±0.17）×10^-3 vs.（1.49±0.08）×10^-3], LC3[（1.9±0.4）×10^-2 vs.（3.8±0.9）×10^-2] mRNA expression in vein graft of WT mice（all P < 0.05）. Compared with the WT mice, there were significant less Atg5 [（0.39±0.05） vs.（0.16±0.08）], LC3[（2.17±0.46） vs.（0.78±0.19）] in vein graft of Atg5^+/- mice（all P < 0.05）. Similarly, the expression of LC3 in smooth muscle cells of Atg5^+/- vein graft was significantly decreased（P<0.05）, and the lumen of vein graft in Atg5^+/- mice was significantly decreased than that in WT mice [（1.63±0.15） ×10⁴ μm² vs.（2.96±0.12）×10⁴ μm², P<0.05]. Conclusions Our results demonstrate that Atg5 deficiency inhibited the stenosis of vein graft.更多还原