【摘要】 目的观察神香草预处理对哮喘小鼠的保护作用,并探讨其可能机制。方法将32只Balb/c小鼠随机分为对照组、哮喘组、地塞米松组及神香草组4组,每组8只。除对照组外,其余3组制备哮喘模型。哮喘组于第1天、第15天腹腔注射0.2 mL致敏液,第22天雾化吸入1%卵清蛋白(OVA)溶液,每次30 min,每周3次,连续8周。地塞米松组和神香草组分别于OVA激发前1 h给予地塞米松0.005 mg/10 g和神香草提取液0.04 g/10 g灌胃,每日1次,连续8周。对照组以生理盐水代替OVA处理。8周后,取材,流式细胞术检测外周血中CD4~+CXCR5~+/CD4~+和CD4~+CXCR5~+ICOS~+/CD4~+CXCR5~+的比例,Real-time PCR检测各组肺组织中BCL-6、CXCR5、ICOS、 ICOSL和IL-21 mRNA的表达,并观察肺组织病理学改变。结果与哮喘组比较,神香草组外周血CD4~+CXCR5~+/CD4~+和CD4~+CXCR5~+ICOS~+/CD4~+CXCR5~+的比例减少,并且肺组织中BCL-6、CXCR5、ICOS、 ICOSL和IL-21 mRNA的表达亦明显降低(均P<0.05)。结论神香草可能通过减少Tfh细胞的数量和影响其相关分子的表达而在慢性哮喘治疗中发挥作用。更多还原

【Abstract】 Objective To observe the protective effect of Hyssopus officinalis L. pretreatment on asthmatic mice and explore its possible mechanism. Methods Thirty two Balb/c mice were randomly divided into 4 groups: control group, asthma group, dexamethasone group and Hyssopus officinalis L. group, with 8 mice in each group. Except for the control group, the other three groups made asthma models. In asthma group, 0.2 ml sensitizing solution was injected intraperitoneally on the 1 st and 15 th days, and 1% ova solution was inhaled on the 22 nd day, 30 minutes each time, three times a week for 8 weeks. Dexamethasone(0.005 mg/10 g) and extract(0.04 g/10 g) were given by gavage one hour before OVA challenge in dexamethasone group and shenvanilla group, respectively, once a day for 8 weeks. The control group was treated with normal saline instead of ova. After 8 weeks, samples were taken and the expression of BCL-6, CXCR5, ICOS, ICOSL and IL-21 mRNAs in lung tissues were detected by real time PCR. Results Compared with the asthmatic group, the percentage of CD4~+CXCR5~+/CD4~+, CXCR5~+ICOS~+/CD4~+CXCR5~+,ICOS~+/CD4~+CXCR5~+ and the expressions of BCL-6, CXCR5, ICOS, ICOSL and IL-21 mRNA in lung tissues were significantly decreased in Hyssopus officinalis L. group(P<0.05).Conclusion Hyssopus officinalis L. may play a therapeutic role in chronic asthma by reducing the number of Tfh cells and affecting the expression of related molecules.更多还原