【摘要】 背景:淫羊藿素作为成骨诱导活性物质已被广泛负载于骨科支架材料中,然而现有研究基本均以淫羊藿素聚合微球形式直接放置于支架孔隙中,导致其释放与支架降解难以同步,且局部有效利用率较低。目的:制备多孔β磷酸三钙-聚吡咯-生物素-淫羊藿素微球复合支架,初步探索该支架与骨髓间充质干细胞共培养的生物学特点。方法:以Fe Cl3为氧化剂,通过氧化化学合成聚吡咯,将聚吡咯、生物素与β磷酸三钙共混合并进行电化学合成,此后采用3D打印技术制备多孔β磷酸三钙-聚吡咯-生物素复合支架,运用HDDD反应制备淫羊藿素-生物素-聚乳酸微球,并将二者组合。将同样3D打印制备的负载淫羊藿素-聚乳酸微球的多孔β磷酸三钙支架设置为对照组。检测并对比两组支架的抗压强度、孔隙率、载药量、药物结合力及药物缓释性能,绘制淫羊藿素释放曲线,扫描电镜观察两组支架对骨髓间充质干细胞的生物学作用。结果与结论:多孔β磷酸三钙-聚吡咯-生物素复合支架的载药量、药物结合力及药物缓释性能显著优于对照组(P <0.05),扫描电镜下支架表面贴壁生长的骨髓间充质干细胞数量显著高于对照组(P <0.05),两组支架抗压强度及孔隙率差异无显著性意义(P> 0.05)。结果表明,多孔β磷酸三钙-聚吡咯-生物素复合支架较传统缓释支架进一步提高了载药能力及缓释性能,并具有良好的力学强度,同时可能具有更好的募集骨髓间充质干细胞参与支架周围骨修复的作用。更多还原

【Abstract】 BACKGROUND: Icaritin, as an osteogenic inducible active substance, has been widely loaded in orthopedic scaffold materials. However, in most of the existing studies, it is mainly placed in the pores of the scaffold directly in the form of polymerized microspheres, which makes it difficult to release icaritin in step with scaffold degradation, and the local effective utilization rate is low. OBJECTIVE: To prepare porous β-tricalcium phosphate-polypyrrole-biotin-icariin microspheres composite scaffolds, and preliminarily explore the biological characteristics of coculture of the scaffolds and bone marrow mesenchymal stem cells. METHODS: Fe Cl3 was used as oxidant, and polypyrrole was synthesized by oxidative chemical synthesis. Polypyrrole, biotin and β-tricalcium phosphate were mixed and electrochemical synthesis was carried out. After that, the porous β-tricalcium phosphate-polypyrrole-biotin composite scaffold was prepared by three-dimensional printing technology. Icariin-biotin-polylactic acid microspheres were prepared by HDDD reaction and loaded on the scaffold above. The ordinary porous β-tricalcium phosphate scaffold prepared by three-dimensional printing was set as the control group and loaded with icaritin-polylactic acid microspheres. The compressive strength, porosity, drug loading, drug binding force and drug sustained release performance of the two groups of scaffolds were detected and compared, and the curve of icaritin release was drawn. The biological effects of the scaffolds on bone marrow mesenchymal stem cells were observed by scanning electron microscopy. RESULTS AND CONCLUSION: The drug loading capacity, drug binding force and drug sustained release performance of the porous β-tricalcium phosphate-polypyrrole-biotin composite scaffold were significantly better than those in the control group(P < 0.05). The number of bone marrow mesenchymal stem cells growing on the surface of the scaffold under scanning electron microscopy was significantly higher than that of the control group(P < 0.05). There was no significant difference in the compressive strength and porosity of the scaffolds between the two groups(P > 0.05). The results confirmed that compared with the traditional sustained-release scaffolds, the porous β-tricalcium phosphate-polypyrrole-biotin composite scaffold further improves the drug loading capacity and sustained-release performance, and has good mechanical strength. Simultaneously, it may have a better role in recruiting bone marrow mesenchymal stem cells to participate in the bone repair around the scaffolds.更多还原