【摘要】 背景:壳聚糖具备优异的理化性能与良好的生物相容性,但其缺乏骨结合的生物活性,需要与其他材料复合用于骨组织修复中。目的:将煅烧骨与壳聚糖复合,分析其理化性能和细胞毒性。方法:采用溶液共混法制备煅烧骨与壳聚糖质量比分别为1/2、1/1、2/1的复合材料,表征3种复合材料的理化性能。在第5代小鼠成纤维细胞L929中分别加入3种复合材料浸提液,CCK-8法检测复合材料的细胞毒性。结果与结论:①X射线衍射和红外光谱显示,3种复合材料的主要成分均为羟基磷灰石与β-磷酸三钙,并且随着煅烧骨比例的增加,复合材料中的羟基磷灰石/β-磷酸三钙的特征衍射峰逐渐增强;②扫描电镜显示,煅烧骨颗粒较均匀地分散于壳聚糖介质中;③随着煅烧骨比例的增加,复合材料的抗压强度逐渐降低;④培养7 d时,3种复合材料浸提液中的细胞生长良好,形态无明显变化;培养9 d的时间内,3种复合材料浸提液中的细胞相对增殖率均在90%以上,细胞毒性均为1级,符合生物材料的安全标准;⑤结果表明,煅烧骨/壳聚糖复合材料具备良好的结构特征、理化性能及合适的抗压强度,并且安全无毒。更多还原

【Abstract】 BACKGROUND: Chitosan exhibits good physiochemical properties and biocompatibility, but it has poor biological activity of osseointegration. Therefore, it needs to combine with other materials for bone repair. OBJECTIVE: Calcined bone/chitosan composite was prepared and its physiochemical properties and biocompatibility were analyzed. METHODS: Calcined bone/chitosan composite was prepared at a mass ratio of 1∶2, 1∶1, 2∶1 respectively by solution blending method. The physicochemical properties of three composite materials were characterized. Passage 5 mouse L929 fibroblasts were treated with the leaching solution of three composite materials. The cytotoxicity of three composite materials was detected by the CCK-8 test. RESULTS AND CONCLUSION:(1) X-ray diffraction and Fourier Transform Infrared Spectroscopy showed that the essential components of three composite materials were hydroxyapatite and β-tricalcium phosphate. The characteristic diffraction peaks of hydroxyapatite/β-tricalcium phosphate increased with the increase of the proportion of calcined bone.(2) Scanning electron microscopy showed that calcined bone particles were evenly dispersed in chitosan medium.(3) With the increase of the proportion of calcined bone, the compressive strength of the composite decreased gradually.(4) After 7 days of culture, the cells in the leaching solution of three composite materials grew well without obvious change in morphology. By the ninth day, the relative proliferation rate of the cells in the leaching solution of three composite materials was over 90%. Cytotoxicity was grade 1, which meets the safety standard of biomaterials.(5) These results suggest that the calcined bone/chitosan composite has good structural characteristics, physicochemical properties and suitable compressive strength and is safe and non-toxic更多还原