【摘要】 该研究利用诱导后的THP-1单核细胞系作为巨噬细胞的模式细胞,鉴定了在巨噬细胞中表达的肌动蛋白亚型,发现β-肌动蛋白和γ-肌动蛋白在巨噬细胞中表达;接着利用共聚焦显微成像发现,β-肌动蛋白和γ-肌动蛋白在巨噬细胞受到脑膜炎大肠杆菌感染时均出现了不同程度的聚集;进一步利用RNA干扰技术实现了对巨噬细胞中的β-肌动蛋白和γ-肌动蛋白的特异性下调,并发现单独下调β-肌动蛋白和γ-肌动蛋白以及联合下调β-和γ-肌动蛋白都使得巨噬细胞吞噬脑膜炎大肠杆菌的能力呈明显下降。这些结果表明,不同亚型的肌动蛋白在巨噬细胞吞噬脑膜炎大肠杆菌过程中发挥着重要作用。更多还原

【Abstract】 In this study,the THP-1 monocytic cells were induced to macrophages and the expression of actin isoforms was analyzed.We found that β-actin and γ-actin were expressed in macrophages.Results from confocal imaging showed that β-actin and γ-actin in macrophages were differentially polymerized upon infection with meningitic E.coli.Then the β-actin and γ-actin in macrophages were specifically downregulated by siRNA-mediated knockdown.We found that downregulation of β-actin and γ-actin resulted in significant reduction in the phagocytosis of meningitic E.coli by macrophages.Our results thus pointed out that different isoforms of actin played an important role during the phagocytosis of meningitic E.coli by macrophages.更多还原