【摘要】 选择七株乳酸菌,富硒培养后对其体外功能特性和抗氧化性进行评价,通过测定无细胞培养物的酶活性确定其抗氧化物质.实验结果表明:七株乳酸菌都有良好的耐硒能力,通过富硒培养其疏水性和抗氧化能力等多种功能特性都有不同程度的提高,胆固醇去除率提高0.11%～13.76%.实验发现富硒后其谷胱甘肽过氧化物酶(Glutathione peroxidase,GSH-Px)活性为7.49±0.63～10.22±0.57 U/mg,硫氧还蛋白还原酶(Thioredoxin reductase,TrxR)活性为3.8±0.47～6.57±0.38 mU/L,两种酶活都有不同程度的提高,由此推测其主要起抗氧化作用的相关酶可能为GSH-Px和TrxR.更多还原

【Abstract】 Seven strains of lactic acid bacteria were selected and cultured in selenium to evaluate their in vitro functional characteristics and antioxidant activity,and the antioxidants were determined by measuring the enzyme activity of cell free extract.The results showed that seven strains of lactic acid bacteria had good selenium tolerance,and their hydrophobicity,antioxidant capacity and other functional characteristics were improved in varying degrees through selenium enrichment culture.The cholesterol removal rate was increased by 0.11%～13.76%.After selenium enrichment,the GSH-Px activity was 7.49±0.63～10.22±0.57 U/mg,and the activity of TrxR was 3.8±0.47～6.57±0.38 mU/L.The activities of the two enzymes were increased to different degrees,so it was speculated that GSH-Px and TrxR were the related enzymes that mainly played an antioxidant role.更多还原