【摘要】 目的探讨miR-23b-3p对CasKi人宫颈癌细胞增殖、侵袭与迁移的影响。方法体外培养CasKi人宫颈癌细胞和HaCaT正常上皮细胞,采用实时定量PCR检测两种细胞miR-23b-3p的表达水平。采用脂质体转染miR-23b-3p模拟物（miR-23b-3p mimic）的方法建立外源性miR-23b-3p过表达的CasKi细胞,阴性对照组中转染无关序列; CCK-8法检测过表达miR-23b-3p后CasKi细胞的增殖, Transwell^TM侵袭实验检测细胞的侵袭能力、 Transwell^TM迁移实验和划痕愈合实验检测CasKi细胞的迁移能力; Western blot法检测神经钙黏蛋白（N-cadherin）、波形蛋白（vimentin）、上皮钙黏蛋白（E-cadherin）、Snail、增殖细胞核抗原（PCNA）、细胞周期蛋白D1（cyclin D1）的蛋白表达。结果与HaCaT细胞相比, CasKi细胞miR-23b-3p表达水平较低。与阴性对照组相比,转染miR-23b-3p mimic的CasKi细胞miR-23b-3p水平明显上调; miR-23b-3p表达上调后,细胞增殖能力减弱, PCNA及cyclin D1表达减少;同时,过表达miR-23b-3p后, CasKi细胞的侵袭及迁移能力均明显受抑制;同时增加E-cadherin蛋白的表达,而抑制vimentin、 Snail和N-cadherin蛋白的表达。结论过表达miR-23b-3p抑制CasKi宫颈癌细胞的增殖、侵袭、迁移和上皮间质转化（EMT）。更多还原

【Abstract】 Objective To investigate the effects of miR-23b-3p on proliferation, migration and invasion of human cervical carcinoma CasKi cells. Methods Human cervical carcinoma CasKi cells and normal epithelial HaCaT cells were cultured in vitro. Real-time quantitative RT-PCR was conducted to detect the expression of miR-23b-3p in CasKi and HaCaT cells. Synthetic miR-23b-3p mimic and its negative control were transfected into CasKi cells by liposome method. The effects of miR-23b-3p over-expression on cell proliferation were detected by CCK-8 assay. Wound scratch healing assay and Transwell^TM assay were used to observe the migration and invasion abilities of CasKi cells, respectively. Western blot analysis was used to detect the protein expression of N-cadherin, vimentin, E-cadherin, Snail, PCNA and cyclin D1. Results The expression of miR-23b-3p in CasKi cells was lower than that of HaCaT cells. Compared with the negative control group, the expression of miR-23b-3p were significantly up-regulated in CasKi cells after transfected with miR-23b-3p mimic. CCK-8 and Western blot assays showed that the proliferation was inhibited and the expression of PCNA and cyclin D1 were down-regulated after the cells were treated with miR-23b-3p mimic. At the same time, after over-expression of miR-23b-3p, the migration and invasion abilities of the CasKi cells were significantly inhibited. In addition, the expression of E-cadherin was up-regulated, while vimentin, Snail and N-cadherin expression levels were significantly down-regulated. Conclusion Over-expression of miR-23b-3p may suppress the proliferation, migration, invasion and epithelial-mesenchymal transition process of human cervical cancer CasKi cells.更多还原