【摘要】 目的:探讨miR-520a在胃癌中的表达以及对胃癌细胞生物学功能的影响。方法:通过real-time PCR检测miR-520a在胃癌组织和胃癌细胞中的表达。采用慢病毒在胃癌细胞SGC7901中过表达miR-520a,在体外观察miR-520a对胃癌细胞增殖、凋亡、侵袭和迁移以及对顺铂(DDP)敏感性的影响。并通过异种移植瘤实验观察过表达miR-520a对胃癌细胞在裸鼠体内生长的影响。结果:与癌旁正常组织相比,miR-520a在胃癌组织中的表达明显降低。与正常胃黏膜上皮细胞GES-1相比,miR-520a在胃癌SGC7901细胞中的表达明显降低,且在顺铂耐药SGC7901/DDP细胞中的表达更低。miR-520a过表达能够促进SGC7901细胞凋亡,抑制细胞增殖、侵袭和迁移。miR-520a过表达促进SGC7901细胞对DDP的药物敏感性增加。异种移植瘤实验发现miR-520a过表达的细胞在裸鼠体内形成的肿瘤生长速度减慢。结论:miR-520a可抑制胃癌发生与发展,提高胃癌细胞对顺铂的敏感性。更多还原

【Abstract】 Objective:To investigate the expression of miR-520 a in gastric cancer and its effect on the biological function in gastric cancer cells.Methods:The expression of miR-520 a in gastric tissues and gastric cells was detected by realtime PCR.Lentivirus was used to over-express miR-520 a in gastric cancer SGC7901 cells,and the effect of miR-520 a on proliferation,apoptosis,invasion and migration of gastric cancer cells and the sensitivity to cisplatin(DDP) was evaluated in vitro.In addition,the effect of miR-520 a over-expression on the growth of xenograft tumor was observed in vivo.Results:The expression of miR-520 a in gastric cancer tissues was significantly lower than that in the adjacent normal tissues.Compared with normal gastric mucosal epithelial GES-1 cells,the expression of miR-520 a was significantly decreased in gastric cancer SGC7901 cells and even lower in cisplatin resistant SGC7901/DDP cells.MiR-520 a over-expression promoted apoptosis and inhibited cell proliferation,invasion and migration in SGC7901 cells.MiR-520 a over-expression promoted the drug sensitivity of SGC7901 cells to DDP.Xenograft tumor experiment data showed that the miR-520 a over-expression slowed down the tumor growth in nude mice.Conclusion:MiR-520 a can inhibit the occurrence and development of gastric cancer and enhance the sensitivity of gastric cancer cells to cisplatin.更多还原