【摘要】 目的:分析人血清和玻璃体炎症相关因子在增生性玻璃体视网膜病变(PVR)发生发展中的作用。方法:46例视网膜疾病患者,依据临床诊断分为视网膜前膜/黄斑裂孔组(对照组,n=15)、孔源性视网膜脱离组(RRD组,n=14)以及PVR组(n=17),在Luminex xMAP平台上采用免疫复合分析法检测炎症相关因子水平,包含4种生长因子[细胞生长因子(EGF)、成纤维细胞生长因子-2(FGF-2)、转化生长因子-α(TGF-α)、血管内皮生长因子(VEGF)]、12种非白介素细胞因子[肿瘤坏死因子-α(TNF-α)、嗜酸性粒细胞趋化因子(Eotaxin)、粒细胞-巨噬细胞集落刺激因子(GM-CSF)、干扰素-α2(IFN-α2)、干扰素-γ(IFN-γ)、肿瘤生长相关因子(GRO)、巨细胞源性趋化因子(MDC)、可溶性白细胞分化抗原40配体(sCD40L)、趋化因子IP-10、单核细胞趋化蛋白1(MCP-1)、单核细胞趋化蛋白1α(MIP-1α)、单核细胞趋化蛋白1β(MIP-1β)]和15种白细胞介素[白细胞介素-1α(IL-1α)、IL-1β、IL-2、IL-3、IL-4、IL-5、IL-6、IL-7、IL-8、IL-9、IL-10、IL-12 p70、IL-13、IL-15、IL-17A],分析患者外周血和玻璃体中炎症相关因子水平差异。结果:共收集46份血清标本和38份玻璃体标本;三组患者血清中31种炎症相关因子水平并没有发生明显变化(P>0.05);对玻璃体中10种不同生长因子和细胞因子检测发现,与对照组比较,PVR组患者PDGF-AA、TGF-α、VEGF、IL-6、IL-8和TNF-β水平显著增高(P<0.01)。结论:相较于外周血,PVR组患者玻璃体中有反映疾病进展的更敏感的炎症相关因子。更多还原

【Abstract】 Objective: To analyze the role of the human serum and vitreous inflammation-related factors in the development of proliferative vitreoretinopathy（PVR）. Method: 46 patients with pars plana vitrectomy were entrolled. Then they were divided into macular hole/epiretinal membrane group（Control, n=15）, rhegmatogenous retinal detachment group（RRD group, n=14）, and proliferative vitreoretinopathy group（PVR group, n=17）. 31 inflammation-related factors including EGF,FGF-2,TGF-α,VEGF,TNF-α,Eotaxin,GM-CSF,IFN-α2,IFN-γ,GRO,MDC,sCD40 L,IP-10,MCP-1,MIP-1α,MIP-1β,IL-1α,IL-1β,IL-2,IL-3,IL-4,IL-5,IL-6,IL-7,IL-8,IL-9,IL-10,IL-12 p70,IL-13,IL-15,IL-17 A,were detected using an immunology multiplex assay on a Luminex^@ xMAP^@ platform. Then the levels of inflammation-related factors were analyzed in serum and vitreous samples. Results: 46 serum samples and 38 vitreous samples were obtained. Thirty-one different growth factors and cytokines were detected in serum samples. However, none of the circulating growth factors and cytokines were found to be different in the three groups. Ten different growth factors and cytokines were measured in the vitreous samples. Compared with contol group the concentration levels of PDGF-AA,TGF-α,VEGF,IL-6,IL-8 and TNF-βwere significantly increased in the vitreous of PVR patients（P<0.01）.Conclusion:Compared with peripheral blood,PVR group had more sensitive inflammatory factors in the vitreous which reflected the progression of the disease.更多还原