【摘要】 目的探讨首发抑郁症患者治疗前后外周血中CD4~+CD25~+CD127~-占CD4~+的比例及Treg细胞与抗抑郁治疗的相关性。方法纳入在大庆市第三医院住院治疗的首发抑郁症患者共20例,均符合《精神障碍诊断与统计手册(第5版)》(DSM-5)关于抑郁障碍的诊断标准,汉密尔顿抑郁量表17项版(HAMD-17)总评分>20分;同期纳入在大庆市第三医院体检中心的健康成人作为对照组,共20例。对抑郁症患者治疗前及治疗12周后分别进行HAMD-17评定。对抑郁症患者治疗前、治疗12周后及健康对照组分别采取其外周血,利用流式细胞术(FCM)测定外周血CD4~+CD25~+CD127~-占淋巴细胞的百分比及CD4~+CD25~+CD127~-占CD4+T细胞的百分比,并作对照分析。结果在CD4~+CD25~+CD127~-/CD4~+的水平上,抑郁症患者治疗前、治疗后均高于健康对照组,差异均有统计学意义(P均<0.01);抑郁症患者HAMD-17评分及血清CD4~+CD25~+CD127~-/CD4~+的水平均下降,差异均有统计学意义(P均<0.05),且抑郁症显效组CD4~+CD25~+CD127~-/CD4~+的变化率与HAMD-17评分减分率呈正相关(r=0.716,P<0.05)。结论抑郁症患者经SSRIs类药物治疗后,外周血CD4~+CD25~+CD127~-/CD4~+水平的降低预示抗抑郁疗效较好,有效的抗抑郁治疗可能与调节T细胞有一定的相关性。更多还原

【Abstract】 Objective To investigate the changes of CD4~+CD25~+CD127-/CD4~+ ratio in peripheral blood of patients with first-episode depre ssion before and after treatment,and to analyze the correlation between Treg cells and antidepressant treatment efficacy.Methods A total of 20 patients with first episode depression in Inpatient Department of the Third Hospital of Daqing who met the diagnostic criteria of Diagnostic and Statistical Manual of Mental Disorders’ fifth edition(DSM-5) for major depressive disorder and scored above 20 points in Hamilton Depression Scale-17 item(HAMD-17) were recruited,meanwhile,another 20 healthy individuals in Physical Examination Center were enrolled as healthy control group.Before and 12 weeks after treatment,HAMD-17 scale was used to assess the depression symptoms of selected patients,and flow cytometry(FCM) was used to detect the CD4~+CD25~+CD127~-Treg/CD4~+T cells ratio in peripheral blood of all selected subjects.Then the comparison analysis was carried out.Results The pre-and post-treatment CD4~+CD25~+CD127~-/CD4~+ levels of depression patients were higher than those of healthy controls(P<0.01).Among depression patients,there was a decrease in HAMD-17 score and serum CD4~+CD25~+CD127-/CD4~+levels after treatment(P<0.05).Moreover,the reduction rate of CD4~+CD25~+CD127~-/CD4~+ of dep re ssion patients with clinical response was positively correlated with the reduction rate of HAMD-17(r=0.716,P<0.05).Conclusion Levels of peripheral blood CD4~+CD25~+CD127~-/CD4~+ of depression patients with 12 weeks of SSRI treatment have a significant decrease,and the antidepressant efficacy may have certain correlations with regulatory T cells.更多还原