【摘要】 [目的]建立重黄温敏凝胶中重楼皂苷VI的含量测定方法,并进行体外释放研究。[方法]采用HPLCELSD法,色谱柱为CAPCELL PAK C8柱(4.6 mm×250 mm,5μm),乙腈(A)-水(B)为流动相,梯度洗脱(0～15 min,25%～35%A;15～35 min,35%～40%A;35～40 min,40%～60%A),柱温25℃,流速1.0 mL/min,进样量10μL,漂移管温度92℃,载气流速2.9 L/min。以体外溶蚀法,对重黄温敏凝胶的累积释放情况,进行考察。[结果]重楼皂苷VI在0.430 0～4.300 0 mg范围内线性良好,lgA=1.796 9lgC+4.398 9 (r~2=0.999 9),定量限为0.054 0 mg,平均回收率为101.70%(RSD=1.17%,n=9),体外释放符合Higuchi方程。[结论]该方法方便、稳定、高效,可用于重黄温敏凝胶体外释放研究。更多还原

【Abstract】 [Objective] To establish a determination method for the content of polyphyllin VI in ChongHuang thermosensitive in situ gel and to study the release in vitro. [Methods] A HPLC-ELSD method was adopeted with the CAPCELL PAK C8 column(4.6 mm×250 mm,5 μm),with the mobile phaseconsisting of acetonitrile(A)-water(B)at the flow rate of 1.0 mL/min(0～15 min,25%～35% A;15～35 min,35%～40%A;35～40 min,40%～60%A),with the column temperature 25 ℃,with the injection volume 10 μL,with the drift tube temperature 92 ℃ and the carrier gas pressure was 2.9 L/min. [Results] The polyphyllin VI has good linearity in the range of 0.430 0～4.300 0 mg,lgA=1.796 9 lgC+4.398 9(R~2=0.999 9),The average recovery rate was 101.70%(RSD=1.17%,n=9). The in vitro release study indicated that drug release of polyphyllin VI was best fitted to Higuchi equation. [Conclusion] The established method is proved to be convenient,stable and efficient.更多还原