【摘要】 课题组前期通过高通量测序技术发现,micro RNAssc-mi R-133a-5p在肥胖猪脂肪组织中表达水平显著下调,推测其可能在脂肪沉积过程中发挥了重要作用。分析ssc-mi R-133a-5p序列保守性,利用Target Scan,mi RDB和mi RWalk在线分析工具预测其候选靶基因,进一步对候选靶基因进行蛋白质互作分析以及KEGG分析,最后将预测的靶基因与课题组前期筛选出的与猪脂肪沉积能力相关的基因取交集。结果表明ssc-mi R-133a-5p候选靶基因PPARGC1A与RORA等,参与AMPK,TNF,与胰岛素分泌等信号通路。结果提示ssc-mi R-133a-5p的候选靶基因可能通过多种信号通路发挥重要作用。文章的结果可为micro RNA参与猪的脂肪生成调节机制提供科学依据。更多还原

【Abstract】 In the early stage of our research group, through high-throughput sequencing technology, it was found that the expression level of microRNAssc-miR-133 a-5 p in the fat tissue of obese pigs was significantly down-regulated. It is speculated that it may play an important role in the process of fat deposition. We Analyzed the conservation of ssc-miR-133 a-5 p sequence, used TargetScan, miRDB and miRWalk online analysis tools to predict its candidate target genes, and further analyzed candidate target genes for protein interaction and KEGG pathway analysis, and finaly predicted the target genes intersection with genes related to pig fat deposition ability screened by the research group in the early stage. The results show that ssc-miR-133 a-5 p candidate target genes PPARGC1 A and RORA are involved in signal pathways such as AMPK, TNF, and insulin secretion. The results suggest that candidate target genes of ssc-miR-133 a-5 p may play an important role through multiple signaling pathways.The results of this study can provide a scientific basis for microRNA to participate in the regulation of pig adipogenesis.更多还原