【摘要】 利用计算机辅助精子分析系统(CASA)结合顶体完整率(NAR)的检查,研究了长白猪0.5 m L细管冻精4种解冻程序后精液的质量,综合评价不同解冻方法。结果表明:1)精子冷冻-解冻后在曲线速度(VCL)、直线速度(VSL)、平均路径速度(VAP)、直线性(LIN)、前向性(STR)、鞭打频率(BCF)以及A+B类精子比例的检测性状上降低,差异极显著(P<0.01);精子头侧摆幅度(ALH)也显著减小(P<0.05),活率(Motile rate)变化差异不显著(P>0.05);2)四组解冻程序中,38℃20 s解冻组的活率(Motile rate,MR)、VSL、VCL、STR、A+B精子比例指标均极显著(P<0.01)优于42℃20 s、50℃15 s、62℃5 s解冻组,VAP、LIN与NAR差异不显著(P>0.05);3)Topsis综合评价的结果表明38℃20 s组在孵育2 h、4 h、6 h之后检测性状优于其他解冻组。由此推论,虽然解冻后精子在运动参数发生较大变化,但是38℃20 s的解冻效果优于其他解冻条件。更多还原

【Abstract】 It was studied that the quality parameters of 0.5 ml straws frozen semen from Landrace boars after 4 different thawing procedures by the computer-aided semen analysis system( CASA) combined with examinations of acrosome integrity rate( NAR), and all different thawing methods were evaluated comprehensively. The results showed that:(I) After sperm freezing and thawing, the values of VCL, VSL, VAP, Lin, STR, BCF and A+ B sperm ratio decreased significantly(p < 0.01); the ALH of sperm decreased extremely significantly(p < 0.05), and the motile rate decreased significantly(p < 0.05) There was no significant difference in rate(p > 0.05);(II) Among the four thawing groups, the motile rate, VSL, VCL, STR, a + B of 38℃-20 s thawing group were most;(III)The results of TOPSIS comprehensive evaluation showed that 38℃-20 s group was better than other thawing groups after incubation for 2 hours, 4 hours and 6 hours. It was concluded that, although the sperm motility parameters changed greatly after thawing, the thawing effect of 38℃-20 s group was better than those of other groups.更多还原