【摘要】 目的:采用核磁共振(~1H-NMR)代谢组学技术探讨疲劳运动及虾青素补充对疲劳运动大鼠血清代谢的影响。方法:将36只雄性SD大鼠随机分为3组:对照组(C)、运动组(E)、运动加给药组(EM)。EM组大鼠按每天6.6 mg/kg体重连续进行虾青素灌胃21天,E组与EM组大鼠进行递增负荷至疲劳的游泳运动。运动后即刻对大鼠进行股动脉取血并离心保存,对血样进行~1H-NMR数据采集与处理后找出差异代谢物,并使用MetPA数据库分析代谢通路,结合KEGG数据库筛选出潜在标志物。结果:1)E组相比C组大鼠血清差异代谢物共15种,其中甘油、柠檬酸、氧化三甲胺(P<0.05)、乳酸、乙酰乙酸(P<0.01)浓度显著升高,葡萄糖、脂质、亮氨酸(P<0.05)、肌酸、异亮氨酸、丙氨酸、缬氨酸、α-酮戊二酸、琥珀酸、甜菜碱(P<0.01)浓度显著降低;15种代谢物共参与20条代谢通路,其中缬氨酸、亮氨酸与异亮氨酸的生物合成,酮体的合成与降解,丁酸代谢,乙醛酸与二羧酸代谢,甘油脂代谢,柠檬酸循环6条通路为疲劳干预潜在的靶标代谢通路,潜在代谢标志物为缬氨酸、亮氨酸、异亮氨酸、乙酰乙酸、琥珀酸、甘油、α-酮戊二酸与柠檬酸;2)EM组相比E组大鼠血清差异代谢物共7种,其中葡萄糖(P<0.05)、亮氨酸、异亮氨酸、缬氨酸(P<0.01)浓度显著升高,脂质(P<0.05)、乳酸、甘油(P<0.01)浓度显著降低;7种代谢物共参与9条代谢通路,其中包括缬氨酸、亮氨酸与异亮氨酸的生物合成,甘油脂代谢2条通路为虾青素干预潜在的靶标代谢通路,潜在标志物为缬氨酸、亮氨酸、异亮氨酸与甘油。结论:1)疲劳运动可通过影响缬氨酸、亮氨酸与异亮氨酸的生物合成,酮体的合成与降解,丁酸代谢,三羧酸循环,乙醛酸与二羧酸代谢及甘油脂代谢等6条代谢通路对大鼠机体产生不利影响;2)疲劳运动前补充虾青素可通过改变其中的缬氨酸、亮氨酸与异亮氨酸的生物合成及甘油脂代谢通路达到促进机体脂质代谢、节约BCAA利用、延缓运动疲劳的作用。更多还原

【Abstract】 Objective: We investigated the effects of astaxanthin supplementation and fatigue exercise on serum metabolism of rats to search for potential markers and analyze metabolic pathways. Methods: 24 male rats were randomly divided into 3 groups: control group(C), exercise group(E), exercise and medicine group(EM). Rats in the EM were given astaxanthin by continuous gavage of 6.6 mg/kg per day for 21 days. Rats in the E and EM were given increased swimming load for three weeks until fatigue. Blood was taken from the femoral artery of the rats immediately after exercise and centrifuged for preservation. 1 H-NMR data of the blood samples were collected and processed, then metabolic differentially expressed substances were obtained. Metabolic pathways were analyzed by MetPA database and potential markers were screened by KEGG database. Results: 1) There were 15 kinds of different metabolites compared group E and C in serum. Among them, the concentrations of glycerin, citric acid, trimethylamine oxide(P<0.05), lactic acid and acetyl acetate(P<0.01) significantly raised, however, the concentrations of glucose, lipid, leucine(P<0.05), creatine, isoleucine, alanine, valine, α-ketoglutaric acid, succinic acid, betaine(P<0.01) significantly reduced; these metabolites involved 20 metabolic pathways, the potential target metabolic pathways of fatigue intervention were valine, leucine and isoleucine synthesis; the synthesis and degradation of ketone body, butyric acid metabolism, glyoxylic acid and dicarboxylic acid metabolism, glycerol metabolism, TCA cycle pathway. And potential markers were valine, leucine, isoleucine, acetoacetic acid, succinic acid, glycerin, α-ketoglutaric acid and citric acid; 2) There were 7 kinds of serum metabolites compared group EM and E. Among them, the concentrations of glucose(P<0.05), leucine, isoleucine and valine(P<0.01) were significantly increased, while the concentration of lipid, lactic acid and glycerin were significantly decreased. these metabolites involved 9 metabolic pathways, among which valine, leucine and isoleucine synthesis and glycerol metabolism are potential target metabolic pathways of astaxanthin intervention, and potential markers were valine, leucine, isoleucine and glycerine. Conclusion: 1) Fatigue exercise had adverse effects on the body of rats through the synthesis of valine, leucine and isoleucine; the synthesis and degradation of ketone bodies; butyric acid metabolism; glyoxylic acid and dicarboxylic acid metabolism; glycerol metabolism; TCA cycle pathway. 2) Astaxanthin supplementation before fatigue exercise can promote lipid metabolism, save BCAA utilization and delay exercise fatigue by changing the synthesis of valine, leucine and isoleucine, as well as the glyceride metabolism pathway.更多还原