【摘要】 MYB转录因子在调控植物适应非生物胁迫,尤其是应答盐胁迫上发挥着重要作用。采用大样本数据定量评价MYB转基因植物在盐胁迫环境条件下的表现,了解其调控机制,能够为耐盐植物品种的培育提供理论依据。搜集筛选2008～2018年间国内外与MYB转录因子和盐胁迫相关研究,采纳70篇文献包含263个相对独立研究数据,应用整合分析方法对不同盐胁迫处理条件下MYB过表达转基因植物及其野生型的生理生化、形态学等16个性状指标进行了比较研究。结果表明:在非盐胁迫处理下,MYB过表达转基因植物与其野生型在15个性状指标上没有表现出显著差异,仅在脯氨酸含量这个指标上表现出显著不同;而在盐胁迫处理下,有10个性状指标表现出显著的差异。MYB转录因子在植株总鲜重、存活率、根长、脯氨酸含量、POD酶活性、发芽率和叶绿素含量中发挥着正向调控作用,而在钠钾离子含量比、钠离子含量、丙二醛含量上发挥着负向调控作用。随着盐胁迫时间增加,MYB转录因子对植物根长的增效作用表现出减弱趋势;当胁迫的时间长于15d,对根长的生长直接显现出减效作用。在短时间盐胁迫处理下,随NaCl浓度的增高,MYB过表达转基因植物较其野生型的根长变长、叶绿素含量增多。MYB转录因子能够显著地提高植物对盐胁迫的耐性,但在调控植物适应盐胁迫的能力上因受处理时间、盐浓度和转基因数目等不同而表现出差异。更多还原

【Abstract】 MYB transcription factors play an important role in regulating plant adaptation to abiotic stress, especially tosalt stress.Using large sample data to quantitatively evaluate the performance of MYB transgenic plants under salt stress environment,andunderstanding its regulation mechanism can provide a theoretical basis for cultivating salt-tolerant plant varieties. Our study conducted a meta-analysis through screening literature related to MYB transcription factors and salt stresspublished domestically and abroad from 2008 to 2018, and finally 70 articlescontaining 263 relative independent studies were collected. The physiological, biochemical and morphological characteristics of transgenic plants with MYB overexpression under different salt stress conditions were quantitatively evaluated compared with wild type plants(16 phenotypic evaluation parameters). The results showed thatonly proline content wassignificantly different between MYB-overexpression transgenic plants and wild type plant under normal growth conditions, while 10 out of the 16 phenotypic evaluation parameters of transgenic plants showed significant differences compare with wild type plants under salt stress. The MYB transcription factors played a positive role in 7 of the 10 evaluation parameters including total fresh weight, survival rate, root length,proline content, POD activity, germination rate, and chlorophyll content, but played a negative role in the other three parameters including Na+/K+mol content ratio, Na+content, and malondialdehyde content. The longer the time of salt stress, the weaker the synergistic effect of transgenic plants on root length.With the treatment timelonger than 15 days, the effect of transgenic plants on root length was decreased. The higher the degree of the NaCl concentration, the greater the synergism of root length and chlorophyll content of MYB transgenic plants compared with wild type plants for short time salt stress.MYB transcription factors can significantly improve the tolerance of plant to salt stress, but the ability of plant adaptation to salt stress was varied due to different treatment time, salt concentration and number of transgenic plants.更多还原