【摘要】 对根癌农杆菌(Agrobacterium tumefaciens)介导转化的机制和已成功应用的食用菌种类、转化的具体过程以及影响转化效率的因素等方面进行综述,总结目前农杆菌介导转化法在食用菌基因功能和外源蛋白表达上的应用,分析农杆菌介导食用菌构建随机插入突变体库等方面的问题,并对食用菌无筛选标记遗传转化的应用前景进行展望,以期为食用菌的转基因研究和工程菌株育种提供参考。更多还原

【Abstract】 This article reviews the mechanism of Agrobacterium tumefaciens-mediated transformation(ATMT), types of edible fungi that have been successfully manipulated by ATMT, the specific process of the transformation, factors that affect transformation efficiency, and current applications of the ATMT method on functional gene analyses and heterologous protein expression in edible fungi. The associated problems, e.g. construction of random insertion mutant library, were also analyzed. Future prospects for transformation free of selection markers were discussed in order to provide relevant references for transgenic research and genetic breeding of edible fungi.更多还原