【摘要】 该文以氰基联苯酚为荧光载体,乙酰基为识别基团,基于水合肼引发的脱保护反应和分子内电荷转移机制（ICT）,研发一种能快速识别N₂H₄的开关型荧光探针。在pH=7.4（含1 mmol/L CTAB）的PBS缓冲溶液中,相对于其他测试的分子或离子,探针化合物对N₂H₄表现出良好的选择性和灵敏度,能在5 min内快速检测识别到N₂H₄。当探针浓度为10.0μmol/L时,探针化合物对N₂H₄的检出限为0.08μmol/L,这个限度符合美国环保局定义的N₂H₄浓度水平。在生理条件下,探针识别N₂H₄可以呈现显著的荧光开启信号。同时,细胞成像实验表明探针可以透过细胞膜与N₂H₄作用,为在线监测提供有效的工具分子。更多还原

【Abstract】 Based on the deprotection reaction initiated by hydrazine and the mechanism of intramolecular charge transfer（ICT）, a switch type fluorescent probe was developed to recognize N₂H₄ rapidly. Compared with other tested molecules and ions, the probe showed good selectivity and sensitivity to N₂H₄ in pH=7.4 PBS buffer solution containing 1 mmol/L CTAB, and it could detect N₂H₄ quickly within 5 min. When the concentration of the probe was 10.0 μmol/L, the detection limit of the probe to N₂H₄ was 0.08 μmol/L, which conforms to the concentration level of N₂H₄ defined by the U.S. Environmental Protection Agency. Under physiological conditions, the probe recognition of N₂H₄ showed a significant fluorescence signal. At the same time, the cell imaging experiments exhibited that the probe could react with N₂H₄ through the cell membrane,providing an effective tool molecule for on-line monitoring.更多还原