【摘要】 目的:检测MYOF(Myoferlin)在卵巢上皮性肿瘤中的表达,并探讨MYOF的表达与卵巢癌患者临床病理参数及其预后的关系。方法:通过基因表达谱的动态分析,选取TCGA和GTEx数据集,分析比较MYOF在正常卵巢组织和卵巢癌组织中的表达情况。免疫组化SP法检测55例卵巢上皮恶性肿瘤、13例卵巢上皮交界性肿瘤、19例卵巢上皮良性肿瘤及14例正常卵巢组织中MYOF的表达情况,利用单因素Cox回归模型分析与卵巢上皮恶性肿瘤临床病理参数及预后的关系。结果:MYOF在卵巢上皮恶性肿瘤阳性率为(98.2%),高于卵巢上皮交界性肿瘤(92.3%)、卵巢上皮良性肿瘤(31.6%)及正常卵巢组织(13.2%)(P均<0.05);MYOF在卵巢上皮恶性肿瘤中高表达率为(76.4%),也显著高于卵巢上皮交界性肿瘤(23.1%)、卵巢上皮良性肿瘤(5.3%)及正常卵巢组织(0%)(P均<0.05)。在卵巢上皮恶性肿瘤中,MYOF的表达随着FIGO分期增加而增加(P均<0. 05)。结论:MYOF的表达可用于卵巢癌的预后标记物。更多还原

【Abstract】 Objective:To investigate the expression and clinical significance of MYOF(Myoferlin)in ovarian epithelial carcinoma and its prognosis.Methods:Through dynamic analysis of gene expression profile,TCGA and GTEx data sets were selected to analyze and compare the expression of MYOF gene in normal ovarian and ovarian cancer tissue.Immunohistochemical method was used to measure the expression of MYOF in tissues from 55 ovarian epithelial carcinoma,13 borderline,19 benign ovarian tumors and 14 normal tissues.Univariate Cox proportional hazard model was statistically assessed to investigate its association with clinicopathological factors and survival of ovarian cancer.Results:MYOF was mainly located in the cytomembrane.The positive rate of MYOF in ovarian epithelial carcinoma was significantly higher than in borderline,benign ovarian tumors and normal tissues(98.2%,92.3%,31.6%,13.2% respectively)(P<0.05).MYOF showed significantly increased expression in ovarian epithelial carcinoma with increasing FIGO stage(P<0.05).MYOF was an independent factor for poor prognosis of ovarian epithelial carcinoma(P<0.05).Conclusion:Our study demonstrates that expression of MYOF is associated with the prognosis of ovarian cancer,it might be a potential prognostic biomarker in OV patients.更多还原