【摘要】 通过研究长链非编码RNA NONHSAT068183(lnc068183)在肾透明细胞癌(ccRCC)患者组织中表达水平的变化,分析其表达水平与ccRCC临床指标间的相关性。实验收集55例ccRCC肿瘤组织及其对应的癌旁正常组织,分别设为ccRCC组和对照组;采用qRT-PCR方法检测lnc068183在ccRCC组及对照组组织中的表达水平,用t检验分析其水平变化与ccRCC患者临床指标间的相关性及意义。与对照组相比,lnc068183在ccRCC组肿瘤组织中的表达水平显著降低(P<0.000 1)。在临床诊断指标中,ccRCC肿瘤组织中lnc068183的表达水平与ccRCC患者的TNM分期(P=0.005)及Fuhrman分级(P=0.015)呈负相关;ROC分析显示lnc068183对ccRCC有较明确的辅助诊断意义。作为抑癌基因,lnc068183在ccRCC中表达下调,可能与ccRCC的发生和发展相关,为肾癌诊治及研究提供了潜在的新线索。更多还原

【Abstract】 The expression level of long non-coding RAN NONHSAT068183(lnc068183) in tumorous tissues from clear cell renal cell carcinoma(ccRCC) was measured in order to investigate the association between lnc068183 level change and ccRCC clinicopathological parameters. Clinical tumorous tissues and paracancerous normal tissues from 55 ccRCC patients were collected and classified as ccRCC group and control group. Lnc068183 level was then determined and quantified by qRT-PCR assay, and t test analysis was performed to reveal the relationship and significance of lnc068183 level change with clinicopathological parameters of ccRCC patients. Compared with the control group, lnc068183 level was significantly decreased(P<0.000 1) in tissues from ccRCC group. Concerning the clinical diagnostic parameters, lnc068183 expression level in tumorous tissues was negatively correlated with TNM stage(P=0.005) and Fuhrman grade(P=0.015) of ccRCC patients. ROC curve showed lnc068183 was in potential diagnostic use in ccRCC diagnosis. As a tumor suppressor gene, the downregulation of lnc068183 in patient tumorous tissue is potentially associated with the occurrence and development of ccRCC, providing a new clue for the diagnosis, treatment and research of renal cancer.更多还原