【摘要】 目的:探讨肉毒碱棕榈酰基转移酶2(CarnitinePalmitoyltransferase2, CPT2)在肝癌细胞迁移侵袭中的调控作用。方法:1).用免疫组化实验,检测62对肝癌癌组织与癌周组织中CPT2表达,以明确肝癌组织细胞中CPT2表达是否发生异常改变。2).细胞划痕实验,在人肝癌细胞HLE中分析干涉CPT2表达对肝癌细胞迁移能力的影响。3).Transwell侵袭实验,在人肝癌细胞HLE中分析干涉CPT2表达对肝癌细胞侵袭能力的影响。结果:1). CPT2主要为胞浆染色且染色呈线粒体蛋白染色典型的颗粒状分布;与癌周组织相比,肝癌组织中CPT2表达显著升高。2).细胞划痕实验证实,干涉人肝癌细胞HLE中CPT2表达后,细胞的相对迁移距离显著变短(si Ctrl VS si CPT2#1 VS si CPT2#2=1.00±0.8 VS 0.67±0.42 VS 0.64±0.31)。3).Transwell侵袭实验证实,干涉人肝癌细胞HLE中CPT2表达后,侵袭至小室底部的细胞数目显著变少(si Ctrl VS si CPT2#1 VS si CPT2#2=23.34±3.51 VS 8.00±2.00 VS8.67±1.53)。结论:CPT2在肝癌细胞中表达显著上调,CPT2表达上调促进了肝癌细胞的迁移与侵袭。更多还原

【Abstract】 Objective: To explore the biological functions of CPT2(Carnitine Palmitoyltransferase2) in the migration and invasion abilities of liver cancer cells. Methods: 1). Immunohistochemistry analysis was applied to evaluate the expression of CPT2 in tumor and adjacent non-tumor cells from 62 patients with liver cancer. 2). Effect of CPT2 knockdown by si RNA on the migration ability in HLE cells. 3). Effect of CPT2 knockdown by si RNA on the invasion ability in HLE cells. Results: 1). CPT2 positive staining is mainly localized in cytoplasm with a granular distribution similar to other of mitochondrial protein. The expression of CPT2 was significantly over-expressed in tumor tissues of liver cancer when compared with their adjacent non-tumor tissues. 2). CPT2 knockdown significantly reduced the distance of the migration of HLE cells(si Ctrl VS si CPT2#1 VS si CPT2#2 = 1.00±0.8 VS 0.67±0.42 VS 0.64±0.31). 3). CPT2 knockdown significantly reduced the number of invaded liver cancer cells in HLE cells(si Ctrl VS si CPT2#1 VS si CPT2#2 = 23.34±3.51 VS 8.00 ±2.00 VS 8.67 ±1.53). Conclusions: CPT2 is significantly up-regulated in liver cancer, which promoted both migration and invasion abilities of liver cancer cells.更多还原