【摘要】 目的:探讨白细胞介素37(IL-37)对氧化低密度脂蛋白(ox-LDL)诱导人冠状动脉内皮细胞(HCAECs)分泌单核细胞趋化蛋白1(MCP-1)的影响。方法:HCAECs经siIL-37 mRNA或IL-37b过表达质粒转染24 h后,用80μg/mL ox-LDL处理24h,同时设置空白对照组、未转染ox-LDL处理组、siRNA阴性对照组、空白质粒组。MTT法检测各组细胞增殖能力,RTPCR法检测各组细胞中MCP-1 mRNA的表达,ELISA法检测各组细胞培养液上清中MCP-1蛋白水平。结果:未转染oxLDL处理组细胞内MCP-1 mRNA的表达与培养液上清中的MCP-1蛋白的分泌量与对照组相比均有升高(P<0.05)。siIL-37mRNA组的MCP-1 m RNA和蛋白水平均较siRNA阴性对照组增加(P<0.01)。IL-37b过表达质粒组的MCP-1 mRNA和蛋白水平均较空白质粒组细胞减少(P<0.05)。结论:IL-37对ox-LDL诱导HCAECs分泌MCP-1有抑制作用。更多还原

【Abstract】 Objective: To investigate the effect of interleukin-37(IL-37) on the secretion of monocyte chemoattractant protein-1(MCP-1) in human coronary artery endothelial cells(HCAECs) induced by oxidizedlow density lipoprotein(ox-LDL). Methods: HCAECs were transfected with siIL-37 mRNA or IL-37 b overexpression plasmid for 24 h, and then treated with 80 μg/mL ox-LDL for 24 h. At the same time, blank control group, non transfected ox-LDL treatment group, siRNA negative control group and blank plasmid group were set up. MTT assay was used to detect cell proliferation, RT-PCR was used to detect MCP-1 mRNA expression, and ELISA method was used to detect MCP-1 protein level in cell culture supernatant. Results: Compared with the control group, the expression of MCP-1 mRNA and the secretion of MCP-1 protein in the supernatant of culture medium in the non transfected ox-LDL group were significantly higher than those in the control group(P<0.05).The mRNA and protein levels of MCP-1 in the siRNA-37 mRNA group were higher than those in the siRNA lnoewgearti vthe acno ntthroosl eg rionu bpl(aPnk< 0 p.l0 a1 s).m iMd CgPr-ou1 p m(RPN<0 A. 0 a5 n).d Cproontecilnu slieovne:l sI iLn-I3 L7-3 ca7 nb ionvheirbeixtp trheses isoenc rpeltiaosnm iodf gMroCuPp-w1 e irne HCAECs induced by ox-LDL.更多还原