【摘要】 探讨太原市冬季大气细颗粒物(PM_2.5)全身系统暴露对雄性SD大鼠5种脏器病理学、氧化应激及炎症反应的影响。于2018年11月至2019年1月于太原市采用IVC通气笼对大鼠进行大气PM_2.5的全身暴露染毒,分别染毒1个月和2个月;同时设立相应空气过滤对照组。采用HE染色方法观察大鼠肝、肾、脾、胃和小肠的组织病理学变化,并测定肝、肾、脾和胃的脏器系数;采用生化方法测定大鼠组织匀浆液中的超氧化物歧化酶（SOD）活性、丙二醛（MDA）含量、白细胞介素-6（IL-6）和肿瘤坏死因子-α（TNF-α）的水平。PM_2.5暴露后大鼠5种组织均体现出不同程度的病理学损伤,MDA含量与对照组相比显著增加。PM_2.5染毒使大鼠肝和肾的脏器系数比对照组显著增高;肾、脾、胃和小肠的SOD活性比相应对照组明显上升;肝和小肠的IL-6和TNF-α水平显著高于对照组。2个月暴露的损伤效应比1个月暴露更为严重。大气PM_2.5全身系统暴露（结合气态污染物）可导致大鼠多器官损害,且随着暴露时间延长而损害加重;肝、肾和小肠对PM_2.5刺激表现敏感。其机制可能与PM_2.5引起的组织MDA含量和炎症因子水平增加有关。更多还原

【Abstract】 To investigate pathology,oxidative stress and inflammatory response of whole body exposure of ambient fine particulate matter(PM_2.5) in winter of Taiyuan in China in five organs of male SD rats in this study,rats were exposed to airborne PM_2.5 using a whole-body inhalation system（individual ventilated cage（IVC） exposure system） for 1 month and 2 months respectively from November 2018 to January 2019 in Taiyuan. Meanwhile,the corresponding control groups（filter air groups） were respectively established. The histopathological changes of liver,kidney,spleen,stomach and small intestine in rats were observed by hematoxylin-eosin（HE） staining,and the organ coefficients of liver,kidney,spleen and stomach were measured. The levels of superoxide dismutase（SOD）,malondialdehyde（MDA）,interleukin-6（IL-6） and tumor necrosis factor-α（TNF-α） in rat tissue homogenates were determined by biochemical methods. PM_2.5 exposure caused pathological damage in varying degrees in five organs in rats,and significantly increased MDA contents compared with the corresponding controls. Organ coefficients of liver and kidney in rats exposed to PM_2.5 were raised relative to the controls. The SOD activities of the kidney,spleen,stomach and small intestine were significantly higher than that of the controls. The levels of IL-6 and TNF-α in the liver and small intestine were obviously elevated compared to the controls. The injury effects of 2-month exposure were more severe than that of 1-month exposure. Whole body exposure to atmospheric PM_2.5（combined with gaseous pollutants） caused multiple organ damage in rats,and such injury aggravated with the prolonged exposure time. Liver,kidney and small intestine were sensitive to PM_2.5 stimulation. Increases of MDA contents and inflammatory factor levels might implicate in the impairment of multiple murine organs induced by PM_2.5.更多还原