【摘要】 为提高屎肠球菌B₁₃乳酸菌素的产量,以接种量、培养基初始pH值和发酵温度为考察因素,在单因素试验的基础上,利用Box-Behnken中心组和试验设计原理进行三因素三水平的响应面优化分析,优化后的最佳培养条件为:接种量4.4%、培养基初始pH 5.3、发酵温度33℃。在此条件下,乳酸菌素的抑菌效价达5.32×10⁸ IU/mL,比优化前提高1.44倍,为屎肠球菌B₁₃乳酸菌素的开发利用提供参考依据。更多还原

【Abstract】 In order to improve the bacteriocin production of Enterococcus faecium B₁₃,inoculum,initial pH of the medium and fermentation temperature was taken as an investigation factor,on the basis of single factor experiment,four factors were designed using Design Expert 8.0.6 Box-Behnken center composite design. The optimal fermentation conditions were inoculum amount of 4.4 %,initial pH of 5.3,fermentation temperature of33 ℃. Under this condition,the bacteriocin inhibitory activity was 5.32 ×10⁸ IU/mL,which was 1.44 times higher than before,it provides a reference for the development and utilization of Enterococcus faecium B₁₃ lactobacillus.更多还原