【摘要】 随着微生物代谢工程技术在合成生物学等领域的广泛应用,该技术已被用于提取多种植物天然产物。花色苷是一种水溶性天然色素,能使花和果实着色,已被广泛应用于食品和化妆品行业。目前,花色苷主要从植物材料中提取,但是提取的花色苷具有纯度低和性质不稳定等缺点,因此利用微生物代谢工程技术替代传统方法提取花色苷具有重要意义。本文阐述了工程菌合成花色苷的研究进展,对酶的选择、运输调节、尿嘧啶核苷-5’-二磷酸葡萄糖供应量的调控以及工艺优化等方面进行综述。对花色苷合成代谢途径的了解将为实现微生物生产花色苷的产业化提供重要信息。更多还原

【Abstract】 Microbial metabolic engineering has seen wide application in synthetic biology and the extraction of naturally occurring phytochemicals. Anthocyanins, a class of water-soluble natural pigments, are primarily responsible for the coloration of terrestrial plants and fruits, and are widely used in the food and cosmetic industries. At present, anthocyanins are mainly extracted from plant materials, being characterized by low product purity and unstable nature. Therefore, microbial metabolic engineering is a significant alternative to the traditional extraction method to produce anthocyanins. Here, we review recent progress in the biosynthesis of anthocyanins using engineered bacteria with respect to enzyme selection, transportation regulation, regulation of uridine-5’-diphosphate glucose supply, and process optimization. Understanding of the anthocyanin biosynthetic pathway will provide important information for the industrialization of microbial anthocyanin production.更多还原